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首页> 外文期刊>Epigenetics & Chromatin >The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo
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The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo

机译：对黄瓜中单性花发育的表观遗传调控的探索

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Background Melon ( Cucumis melo ) is an important vegetable crop from the Cucurbitaceae family and a reference model specie for sex determination, fruit ripening and vascular fluxes studies. Nevertheless, the nature and role of its epigenome in gene expression regulation and more specifically in sex determination remains largely unknown. Results We have investigated genome wide H3K27me3 and H3K9ac histone modifications and gene expression dynamics, in five melon organs. H3K9ac and H3K27me3 were mainly distributed along gene-rich regions and constrained to gene bodies. H3K9ac was preferentially located at the TSS, whereas H3K27me3 distributed uniformly from TSS to TES. As observed in other species, H3K9ac and H3K27me3 correlated with high and low gene expression levels, respectively. Comparative analyses of unisexual flowers pointed out sex-specific epigenetic states of TFs involved in ethylene response and flower development. Chip-qPCR analysis of laser dissected carpel and stamina primordia, revealed sex-specific histone modification of MADS-box genes. Using sex transition mutants, we demonstrated that the female promoting gene, CmACS11 , represses the expression of the male promoting gene CmWIP1 via deposition of H3K27me3. Conclusions Our findings reveal the organ-specific landscapes of H3K9ac and H3K27me3 in melon. Our results also provide evidence that the sex determination genes recruit histone modifiers to orchestrate unisexual flower development in monoecious species.
机译：背景甜瓜（Cucumis melo）是葫芦科重要的蔬菜作物，是用于性别确定，果实成熟和血管通量研究的参考模型。然而，其表观基因组在基因表达调节中，更具体地说在性别确定中的性质和作用，仍然是未知的。结果我们研究了五个甜瓜器官中的全基因组H3K27me3和H3K9ac组蛋白修饰和基因表达动态。 H3K9ac和H3K27me3主要分布在基因富集区域，并局限于基因体。 H3K9ac优先位于TSS，而H3K27me3从TSS到TES均匀分布。如在其他物种中观察到的，H3K9ac和H3K27me3分别与高和低基因表达水平相关。单性花的比较分析指出了参与乙烯反应和花发育的TFs的性别特异性表观遗传状态。激光解剖的心皮和耐力原基的芯片qPCR分析，揭示了MADS-box基因的性别特异性组蛋白修饰。使用性别过渡突变体，我们证明了女性促进基因CmACS11通过沉积H3K27me3抑制了男性促进基因CmWIP1的表达。结论我们的发现揭示了瓜中H3K9ac和H3K27me3的器官特异性景观。我们的研究结果还提供了证据，表明性别决定基因会募集组蛋白修饰剂，以协调单性物种中单性花的发育。

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