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首页> 外文期刊>Eurasian Journal of Analytical Chemistry >Validated Absorption Factor Spectrophotometric and Reversed-Phase High-Performance Liquid Chromatographic Methods for the Determination of Ramipril and Olmesartan Medoxomil in Pharmaceutical Formulations
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Validated Absorption Factor Spectrophotometric and Reversed-Phase High-Performance Liquid Chromatographic Methods for the Determination of Ramipril and Olmesartan Medoxomil in Pharmaceutical Formulations

机译：经验证的吸收系数分光光度法和反相高效液相色谱法测定药物制剂中的雷米普利和奥美沙坦

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Two simple and sensitive spectrophotometric and liquid chromatographic (LC) methods are described for the determination of ramipril and olmesartan medoxomil. The first method was based on the absorption factor. Ramipril and olmesartan medoxomil exhibit λmax at 210 nm and 256 nm respectively. Olmesartan medoxomil has some interference at 210nm, while ramipril do not show any absorption at 256 nm.Quantitative estimation of ramipril was carried out by subtracting the absorption due to olmesartan medoxomil at 210 nm using experimentally calculated absorption factor. Beer’s law was obeyed for ramipril and olmesartan medoxomil at 2-6 µg mL-1 and 8-24 µg mL-1 respectively. The second method, high-performance liquid chromatographic method was developed for the determination of ramipril and olmeasartan medoxomil using sodium perchlorate: acetonitrile (60:40, v/v) as the mobile phase and measuring the response at λmax 210 nm. The analysis was performed on a Phenomenex C8 (250 X 4.0 mm), 5 µm column. The calibration curve was obtained for ramipril and olmesartan medoxomil at 1-6 µg mL-1 and 4-24 µg mL-1 respectively. The mean recovery was 99.80 ± 0.20% and 99.86 ± 0.20% for ramipril and olmesartan medoxomil respectively. The methods were validated according to the ICH guidelines.
机译：描述了两种简单而灵敏的分光光度法和液相色谱法（LC），用于测定雷米普利和奥美沙坦酯。第一种方法基于吸收系数。雷米普利和奥美沙坦美多西米分别在210 nm和256 nm处表现出λ max 。 olmesartan medoxomil在210nm处有一些干扰，而ramipril在256 nm处无吸收。使用实验计算的吸收系数减去olmesartan medoxomil在210 nm处的吸收，对ramipril进行定量估计。雷米普利和奥美沙坦美多米分别以2-6 µg mL -1 和8-24 µg mL -1 遵守比尔定律。第二种方法是高效液相色谱法，以高氯酸钠：乙腈（60:40，v / v）为流动相并在λ max处测量响应，用于测定雷米普利和奥美沙坦美多佐米的含量。子> 210 nm。在Phenomenex C 8 （250 X 4.0 mm），5 µm色谱柱上进行分析。雷米普利和奥美沙坦美多米分别在1-6μgmL -1 和4-24μgmL -1 下获得校准曲线。雷米普利和奥美沙坦medoxomil的平均回收率分别为99.80±0.20％和99.86±0.20％。该方法已根据ICH指南进行了验证。

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