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首页> 外文期刊>Eurasian Journal of Analytical Chemistry >A Stability-Indicating HPLC Method for Cefoperazone
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A Stability-Indicating HPLC Method for Cefoperazone

机译：头孢哌酮的稳定性指示HPLC方法

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摘要

Stability indicating HPLC assay method for the standard drug cefoperazone was developed using reverse phase appurassil C-18, 250 X 4.6mm. 5μm column, in the mobile phase phosphate buffer (pH 6.8) and methanol (5:2) at flow rate 1mLmin-1 with UV detection at 254 nm. The retention time was found to be 2.67 min. Validation of an analytical method was established by laboratory studies. The proposed method was found to be linear at concentration of 1 to 10 μgmL-1 (R2=0.9899). The limit of detection and limit of quantification was 0.2 μgmL-1 and 0.4 μgmL-1 respectively and the method was found to be specific with respect to specificity. Method precision and precision of the system was found to be within the limits of the acceptance criteria. Relative Standard deviation for precision of the method and precision of the system was found to be 0.49% and 0.7451% respectively. The percentage recovery ranges from 95 –106 % The results indicate that there is no interference from excipients for the proposed method, thus making the method more simple, less time consuming and suitable for routine quantitative estimation of cefoperazone sodium injection formulation. As the method could effectively separate the drug from its degradation products, it can be employed as a stability indicating one.
机译：使用250×4.6mm反相装置C-18开发了用于标准药物头孢哌酮的稳定性指示HPLC测定方法。 5μm色谱柱，在流动相磷酸盐缓冲液（pH 6.8）和甲醇（5：2）中，流速1mLmin-1，在254 nm处进行UV检测。发现保留时间为2.67分钟。通过实验室研究确定了分析方法的有效性。发现所提出的方法在1至10μgmL-1的浓度下是线性的（R2 = 0.9899）。检测极限和定量极限分别为0.2μgmL-1和0.4μgmL-1，并且发现该方法具有特异性。方法的精度和系统的精度被认为在接受标准的范围内。方法精度和系统精度的相对标准偏差分别为0.49％和0.7451％。回收率范围为95 –106％。结果表明，所提议的方法没有辅料的干扰，因此使该方法更简单，耗时更少，并且适合于头孢哌酮钠注射剂的常规定量估计。由于该方法可以有效地将药物与其降解产物分离，因此可以用作一种指示稳定性的药物。

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