首页> 外文期刊>African Journal of Microbiology Research >Bacteriostatic or bactericidal action of four aqueous plant extracts on multi-drug resistant bacteremia and their effect on cells morphology recorded using scanning electron microscopy (SEM)
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Bacteriostatic or bactericidal action of four aqueous plant extracts on multi-drug resistant bacteremia and their effect on cells morphology recorded using scanning electron microscopy (SEM)

机译:使用扫描电子显微镜(SEM)记录的四种水性植物提取物对多药耐药菌血症的抑菌或杀菌作用及其对细胞形态的影响

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Four aqueous traditional plant extracts from rosemary leaves (Rosmarinus officinalis), clove buds (Syzygium aromaticum [L.] Merr at Perry, Myrtaceae), cinnamon bark (Cinnamomum zeylanicum) and ginger rhizomes (Zingiber officinale) were tested as natural antibiotics against multi-drug resistant bacteremia isolates: Six Gram negative isolates, viz. Acinetobacter baumanni/haemolyticus, Citrobacter freundii, Escherichia coli, E. coli ESβL, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella sp. and four Gram positive isolates viz. methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Streptococcus pneumoniae and Bacillus sp. were which used to test antibiotic activity. The usage of clove extract (25X) appeared to have maximum antibacterial activity against all tested Gram negative bacteria, when compared with other tested plant extracts at the same concentration. In the case of G+ve bacteria, tested extracts appeared to have almost the same efficiency. Swabs were taken from inhibition zone resulting from the inhibitory effect of aqueous extracts towards the tested isolates and re-inoculated to detect either the bacteriostatic or bactericidal action. Again, clove extract exhibited the highest effect when compared with other tested against the isolates from bloodstream. Scanning electron microscopy was used to record external morphological changes of the bacterial cells such as appendages, shortness and lyses.
机译:测试了从迷迭香叶(Rosmarinus officinalis),丁香花蕾(Syzygium aromaum [L.] Merr at Myrtaceae),肉桂皮(Cinnamomum zeylanicum)和姜根茎(Zingiber officinale)四种天然植物提取物作为天然抗生素对多种农药的抗药性。耐药菌血症分离株:六种革兰氏阴性分离株,即。鲍曼不动杆菌/溶血性杆菌,弗氏柠檬酸杆菌,大肠杆菌,大肠杆菌ESβL,肺炎克雷伯菌,铜绿假单胞菌,沙门氏菌。和四个革兰氏阳性分离株。耐甲氧西林的金黄色葡萄球菌(MRSA),表皮葡萄球菌,肺炎链球菌和芽孢杆菌。是用来测试抗生素活性的。与相同浓度的其他受试植物提取物相比,丁香提取物(25X)的使用似乎具有对所有受试革兰氏阴性细菌最大的抗菌活性。对于G + ve细菌,经测试的提取物似乎具有几乎相同的效率。从水提取物对被测分离物的抑制作用产生的抑制区域中取出拭子,并再次接种以检测抑菌或杀菌作用。再次,丁香提取物与其他针对血流分离物的测试相比,显示出最高的效果。扫描电子显微镜用于记录细菌细胞的外部形态变化,例如附肢,短小和裂解。

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