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Isolation and characterization of rhamnolipid producing Pseudomonas aeruginosa strains from waste edible oils

机译:从废食用油中分离生产鼠李糖脂的绿脓杆菌铜绿假单胞菌菌株

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This study?was?to screen efficient biosurfactant producing strains from waste edible oils (WEOs). Two strains Y5 and Y17 were screened out by steps of preliminary blood plate screening and secondary flask fermentation screening. The surface tension of the fermentation broth of the two strains diluted by 100 times was lower than 40 mN/m. Preliminary characterization of fermentation products was conducted, which was found to be rhamnolipid ultimately. Y5 strain produced only one kind of glycolipids while the Y17 produced at least three kinds of homologues of glycolipids.?Both?Y5 and Y17 were identified as?Pseudomonas aeruginosa?strains.?This study gave?a?simple and efficient protocol to screen out biosurfactant producing strains and?would?pave the way for the production of biosurfactant from WEOs.
机译:这项研究是从废食用油(WEOs)中筛选出高效生产生物表面活性剂的菌株。通过初步的血液板筛选和二级烧瓶发酵筛选步骤筛选出两个菌株Y5和Y17。稀释100倍的两个菌株的发酵液的表面张力低于40mN / m。对发酵产物进行了初步表征,最终发现其为鼠李糖脂。 Y5菌株仅产生一种糖脂,而Y17产生至少三种糖脂的同源物。“ Y5和Y17都被鉴定为铜绿假单胞菌”菌株。这项研究给出了“简单有效的筛选方案”生产生物表面活性剂的菌株,并将为从WEO生产生物表面活性剂铺平道路。

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