Identifying bacteria and studying bacterial diversity using the 16S ribosomal RNA gene-based sequencing techniques: A review

Khayalethu Ntushelo

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Identifying bacteria and studying bacterial diversity using the 16S ribosomal RNA gene-based sequencing techniques: A review

机译：使用基于16S核糖体RNA基因的测序技术鉴定细菌并研究细菌多样性：综述

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This study reflects the usefulness of sequence analysis of the 16S ribosomal RNA gene in identifying bacteria and determining bacterial diversity. Various techniques that are based on utilizing the 16S rRNA gene are discussed. Of critical importance is the use of massively parallel sequencing to study bacterial diversity. Through massively parallel sequencing which is replacing traditional methods of bacterial identification; various bacterial habitats are surveyed to compile their species compositions.

机译：这项研究反映了16S核糖体RNA基因序列分析在鉴定细菌和确定细菌多样性中的有用性。讨论了基于利用16S rRNA基因的各种技术。至关重要的是使用大规模平行测序来研究细菌多样性。通过大规模并行测序，取代了传统的细菌鉴定方法;调查了各种细菌栖息地，以汇编其物种组成。

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《African Journal of Microbiology Research》 |2013年第49期|共8页
作者
Khayalethu Ntushelo;
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中图分类微生物学;
关键词
Bacterial identification16S ribosomal RNA geneDNA;

机译：细菌鉴定16S核糖体RNA基因DNA;

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1. Identifying bacteria and studying bacterial diversity using the 16S ribosomal RNA gene-based sequencing techniques: A review [J] . Khayalethu Ntushelo African Journal of Microbiology Research . 2013,第49期

机译：使用基于16S核糖体RNA基因的测序技术鉴定细菌并研究细菌多样性：综述
2. Diagnostic accuracy of a 16S ribosomal DNA gene-based molecular technique (RT-PCR, microarray, and sequencing) for bacterial meningitis, early-onset neonatal sepsis, and spontaneous bacterial peritonitis. [J] . Esparcia O, Montemayor M, Ginovart G, Diagnostic microbiology and infectious disease . 2011,第2期

机译：基于16S核糖体DNA基因的分子技术（RT-PCR，微阵列和测序）对细菌性脑膜炎，早发性新生儿败血症和自发性细菌性腹膜炎的诊断准确性。
3. Use of the MicroSeq 500 16S rRNA Gene-Based Sequencing for Identification of Bacterial Isolates That Commercial Automated Systems Failed To Identify Correctly [J] . Carla Fontana, Marco Favaro, Marco Pelliccioni, Journal of Clinical Microbiology . 2005,第2期

机译：基于MicroSeq 500 16S rRNA基因的测序用于细菌分离物鉴定的商业自动化系统无法正确鉴定的细菌分离物
4. Massive multiple sequence alignment of 16S bacterial ribosomal RNAs using ClustalW-Message Passing Interface (MPI) Based on Beowulf Linux system [C] . Hyon Chang Kim, Yong Beom Seo, Ji Hwan Song, . 2005

机译：基于Beowulf Linux系统的ClustalW-消息传递接口（MPI）对16S细菌核糖体RNA进行大规模多序列比对
5. An integrated investigation of ruminal microbial communities using 16S rRNA gene-based techniques [D] . Kim, Min Seok 2011

机译：使用基于16S rRNA基因的技术对瘤胃微生物群落进行综合研究
6. Effect of Field Inoculation with Sinorhizobium meliloti L33 on the Composition of Bacterial Communities in Rhizospheres of a Target Plant (Medicago sativa) and a Non-Target Plant (Chenopodium album)—Linking of 16S rRNA Gene-Based Single-Strand Conformation Polymorphism Community Profiles to the Diversity of Cultivated Bacteria [O] . Frank Schwieger, Christoph C. Tebbe 2000

机译：苜蓿中华根瘤菌L33田间接种对目标植物（Medicago sativa）和非目标植物（Chenopodium album）的根际细菌群落组成的影响—基于16S rRNA基因的单链构象多态性社区概况与链接培养细菌的多样性
7. Use of the MicroSeq 500 16S rRNA gene-based sequencing for identification of bacterial isolates that commercial automated systems failed to identify correctly [O] . Fontana, C, Favaro, M, Pelliccioni, M, 2005

机译：基于MicroSeq 500 16S rRNA基因的测序用于鉴定商业自动化系统无法正确鉴定的细菌分离株

Identifying bacteria and studying bacterial diversity using the 16S ribosomal RNA gene-based sequencing techniques: A review

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