...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Microbiology Research >Isolation and identification of Escherichia coli and Edwardsiella tarda from fish harvested for human consumption from Zeway Lake, Ethiopia
【24h】

Isolation and identification of Escherichia coli and Edwardsiella tarda from fish harvested for human consumption from Zeway Lake, Ethiopia

机译：从埃塞俄比亚泽威湖收获的人类食用鱼中分离和鉴定大肠杆菌和塔氏爱德华氏菌

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A cross sectional study was conducted from November to June 2014/2015 in fish harvested for human consumption at Lake Zeway with the objective of isolating Edwardsiella tarda and Escherichia coli important pathogens and contaminants its products. Three hundred tissue samples comprising Spleen, Liver and Intestine were collected from 100 fish (12 Clarias gariepenus and 88 Oreochromis niloticus) originated from lake Zeway. Escherchia coli were isolated from spleen, liver, and the intestine of 12 out of 100 (12%) while E. tarda was isolated 4 out of 100 (4%) randomly examined fish. The higher percentage of both bacteria was isolated from intestinal samples than those isolated from liver and spleen samples. The E.tarda isolates appeared as small scatter pinkish colonies with black center while isolated colonies from E. coli were yellowish white on Xylose deoxycholate agar (XLD) after 24 h of incubation at 37°C Both types of isolates were gram negative short to medium rods, motile, catalase positive and oxidase negative. In biochemical test, both of the two types of typical isolates were positive for indole and Methyl red test and negative for Vagos proskaur test and unable to utilize Simmon’s citrate test. In addition to this, E. tarda ferments sodium thiosulfate and result its byproduct of H2S production (blackening) on TSI test which were absent in E.coli. The distribution of E.tarda and E.coli infection among the three organs examined indicate both bacteria were isolated most frequently from intestine followed by spleen and liver with significance difference (P 0.05) indicating that sex has no role in influencing the exposure these bacteria. The isolation of E.tarda and Escherchia coli from wild fish population of Lake Zeway destined for human consumption in the current study indicated that E. tarda and Escherchia coli are a potential threat for fish and public health.Key word: Catfish, Escherchia coli, Edwardsiella tarda, intestine, liver, spleen tilapia, zeway.
机译：从2014年11月至2014/2015年6月，对在泽威湖供人类食用的鱼类进行了横断面研究，目的是分离塔氏爱德华氏菌和大肠杆菌的重要病原体及其污染物。从来自Zeway湖的100条鱼（12条Cl鱼和88条尼罗罗非鱼）中收集了包含脾，肝和肠的三百个组织样本。从脾脏，肝脏和肠道中分离出大肠埃希氏菌，每100条中有12条（占12％），而从100条随机检查的鱼中，有tarda大肠杆菌中有4种（4％）从肠中分离出来。从肠道样品中分离出的两种细菌的​​百分比均高于从肝脏和脾脏样品中分离出的两种细菌。在37°C孵育24小时后，在大肠杆菌中分离的小菌落呈粉红色，中心呈黑色，呈粉红色小菌落，而从大肠杆菌分离的菌落在木糖脱氧胆酸琼脂（XLD）上呈黄白色。棒，运动，过氧化氢酶阳性和氧化酶阴性。在生化测试中，两种典型分离株的吲哚和甲基红测试均为阳性，而瓦格斯proskaur测试均为阴性，无法利用Simmon的柠檬酸盐测试。除此之外，焦油大肠埃希氏菌发酵硫代硫酸钠，并通过TSI测试得出其硫化氢产生的副产物（变黑），而大肠杆菌中不存在。大肠杆菌和大肠杆菌感染在三个器官中的分布情况表明，这两种细菌最常从肠道中分离出来，其次是脾脏和肝脏，差异具有显着性（P 0.05），表明性别对这些细菌的暴露没有影响。目前的研究表明，从泽韦湖野生鱼类种群中分离出人类食用的大肠埃希氏菌和大肠埃希氏菌，表明大肠埃希氏菌和大肠埃希氏菌是对鱼类和公众健康的潜在威胁。关键词：Cat鱼，大肠埃希氏菌，爱德华氏菌，肠，肝脏，罗非鱼脾，zeway。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号