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首页> 外文期刊>European Cells & Materials >Enhanced in vitro biological activity generated by surface characteristics of anodically oxidized titanium – the contribution of the oxidation effect
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Enhanced in vitro biological activity generated by surface characteristics of anodically oxidized titanium – the contribution of the oxidation effect

机译：阳极氧化钛的表面特性产生的增强的体外生物活性–氧化作用的贡献

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Anodically oxidized titanium surfaces, prepared by spark discharge, have micro-submicron surface topography and nano-scale surface chemistry, such as hydrophilic functional groups or hydroxyl radicals in parallel. The complexity of the surface characteristics makes it difficult to draw a clear conclusion as to which surface characteristic, of anodically oxidized titanium, is critical in each biological event. This study examined the in vitro biological changes, induced by various surface characteristics of anodically oxidized titanium with, or without, release of hydroxyl radicals onto the surface. Anodically oxidized titanium enhanced the expression of genes associated with differentiating osteoblasts and increased the degree of matrix mineralization by these cells in vitro. The phenotypes of cells on the anodically oxidized titanium were the same with, or without, release of hydroxyl radicals. However, the nanomechanical properties of this in vitro mineralized tissue were significantly enhanced on surfaces, with release of hydroxyl radicals by oxidation effects. In addition, the mineralized tissue, produced in the presence of bone morphogenetic protein-2 on bare titanium, had significantly weaker nanomechanical properties, despite there being higher osteogenic gene expression levels. We show that enhanced osteogenic cell differentiation on modified titanium is not a sufficient indicator of enhanced in vitro mineralization. This is based on the inferior mechanical properties of mineralized tissues, without either being cultured on a titanium surface with release of hydroxyl radicals, or being supplemented with lysyl oxidase family members.
机译：通过火花放电制备的阳极氧化钛表面具有微米-亚微米级的表面形貌和纳米级的表面化学性质，例如平行的亲水性官能团或羟基自由基。表面特性的复杂性使得很难得出清晰的结论，即阳极氧化钛的哪种表面特性在每个生物事件中都是至关重要的。这项研究检查了由阳极氧化的钛的各种表面特性引起的体外生物学变化，无论有无羟基释放到表面上。阳极氧化钛增强了与分化成骨细胞相关的基因的表达，并增加了这些细胞在体外的基质矿化程度。在阳极氧化的钛上，细胞的表型相同，有或没有释放羟基。但是，这种体外矿化组织的纳米机械性能在表面上得到了显着增强，并通过氧化作用释放了羟基自由基。另外，尽管成骨基因表达水平较高，但在裸钛上存在骨形态发生蛋白2的情况下产生的矿化组织的纳米力学性能明显较弱。我们表明，在改性钛上增强的成骨细胞分化不是增强体外矿化作用的充分指标。这是基于矿化组织的较差的机械性能，而不是在释放羟基自由基的钛表面上进行培养，或者未添加赖氨酰氧化酶家族成员。

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