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首页> 外文期刊>European Cells & Materials >Intraosseous transcutaneous amputation prostheses versus dental implants: A comparison between keratinocyte and gingival epithelial cell adhesion in vitro
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Intraosseous transcutaneous amputation prostheses versus dental implants: A comparison between keratinocyte and gingival epithelial cell adhesion in vitro

机译：骨内经皮截肢假体与牙种植体：体外角质形成细胞和牙龈上皮细胞粘附的比较

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Infection is the primary failure modality for transcutaneous implants because the skin breach provides a route for pathogens to enter the body. Intraosseous transcutaneous amputation prostheses (ITAP) are being developed to overcome this problem by creating a seal at the skin-implant interface. Oral gingival epithelial cell attachment creates an infection-free seal around dental implants. However, this has yet to be achieved consistently outside of the oral environment. Epithelial cells attach to metal substrates by means of hemidesmosomes and focal adhesions. Their density per unit cell is an indicator of attachment strength. We postulate that gingival epithelial cells express more hemidesmosomes and focal adhesions at earlier time points, compared with epidermal keratinocytes, and this increased speed and strength of attachment may be the reason why an infection-free seal is often achieved around dental implants but less frequently around ITAP. The aim of this study was to compare epidermal keratinocyte with oral gingival cell attachment on titanium alloy in vitro, to determine whether these two cell types differ in their speed and strength of attachment. We aimed to test the hypothesis that gingival cells up-regulate focal adhesion and hemidesmosome formation at earlier time points compared with extra-oral keratinocytes. To test this hypothesis we cultured epidermal keratinocytes and oral gingival cells on titanium alloy substrates and assessed cell attachment by focal adhesions and hemidesmosome expression at 4, 24, 48 and 72 hours. Formation and expression of hemidesmosomes temporally lagged behind that of focal adhesions in both cell types. Gingival derived cells up-regulated focal adhesion and hemidesmosome expression at earlier time points compared with epidermal keratinocytes. Hemidesmosome expression in oral gingival cells was 3 times greater compared with epidermal keratinocytes at 4 hours. Our findings indicate that earlier attachment may be key to the success of the dental implant transcutaneous interface.
机译：感染是经皮植入物的主要失败方式，因为皮肤破裂为病原体进入人体提供了途径。骨内经皮截肢假体（ITAP）的开发是为了通过在皮肤-植入物界面处形成密封来克服这一问题。口腔龈上皮细胞附着可在牙科植入物周围形成无感染的密封。然而，这仍需要在口腔环境之外始终实现。上皮细胞通过半脂质体和粘着斑附着在金属基质上。它们的每晶胞密度是附着强度的指标。我们推测，与表皮角质形成细胞相比，牙龈上皮细胞在更早的时间点表达更多的半脂质体和粘着斑，这种附着的速度和强度的提高可能是为什么在种植牙周围通常实现无感染密封但在种植体周围较少发生密封的原因ITAP。这项研究的目的是比较表皮角质形成细胞与钛合金上口腔牙龈细胞的体外附着力，以确定这两种细胞的附着速度和强度是否不同。我们的目的是检验与口腔外角质形成细胞相比，牙龈细胞在更早的时间点上调粘着斑粘附和血小体形成的假说。为了验证该假设，我们在钛合金基底上培养了表皮角质形成细胞和口腔牙龈细胞，并在4、24、48和72小时通过黏着斑和半小体表达评估了细胞附着。在这两种细胞类型中，半桥粒的形成和表达在时间上均落后于粘着斑。与表皮角质形成细胞相比，牙龈衍生细胞在较早的时间点上调了局部黏附和半小体的表达。与表皮角质形成细胞相比，在4小时时，口腔牙龈细胞中的半脂质体表达高3倍。我们的发现表明，较早的附着可能是牙种植体经皮界面成功的关键。

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