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首页> 外文期刊>European Chemical Bulletin >OPTIMIZATION OF EXTRACTION CONDITIONS FOR LIQUID-LIQUID EXTRACTION OF PERSIPEPTIDES FROM STREPTOMYCES ZAGROSENSIS FERMENTATION BROTH
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OPTIMIZATION OF EXTRACTION CONDITIONS FOR LIQUID-LIQUID EXTRACTION OF PERSIPEPTIDES FROM STREPTOMYCES ZAGROSENSIS FERMENTATION BROTH

机译：从ZAGROSENSIS发酵液发酵液中提取杀虫肽的液体条件的优化

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摘要

Generally, during drug discovery programs, after identification of new antibiotic metabolite, its high quantity production is obtained by various approaches, including production or extraction improvement or even strain genetic manipulation. To provide enough amounts of two novel non-toxic anti-MRSA pentapeptides named persipeptides (A and B) required for drug discovery programs, seven different fermentation broths examined. CM1 medium considerably enhanced the biosynthesis of persipeptides up to 219.63 ± 2.48, compared with ISP2 medium (36.31 ± 1.37), showing a six-fold increase. Additionally, at the extraction level, results of experimental design indicated that liquid-liquid extraction (LLE) of persipeptides by 34 % BuOH at 228 rpm (Stirrer speed), temperature 28 ?C, and pH 9-9.5 for 78 min (stirring time) was equal to 264 ± 9.85 μg mL -1 , which was the most favorable combination for their extraction. Compared with un-optimized extraction process (219.63 ± 2.48 μg mL -1 ), the optimized conditions improved the yield of the extraction by 20.20 %, while saving both time and solvent usage up to 67 % (162 min) and 16 %, respectively. The total sum of persipeptides enhancements resulted from the replacement of fermentation broth and subsequent optimization of their extraction by LLE reached almost seven-time, compared to conventional method (36.31 ± 1.37 μg mL -1 ). Therefore, relatively large amounts of persipeptides can be economically produced and extracted for various future experiments.
机译：通常，在药物开发计划中，在鉴定出新的抗生素代谢物后，可通过多种方法获得大量生产，包括提高产量或提取率，甚至进行菌株遗传操作。为了提供足够数量的两种新的无毒抗MRSA五肽五肽（称为过肽（A和B）），这是药物发现计划所需的，我们检查了七个不同的发酵液。与ISP2培养基（36.31±1.37）相比，CM1培养基显着提高了脂肽的生物合成，至219.63±2.48，增加了6倍。此外，在提取水平上，实验设计结果表明，在228 rpm（搅拌速度），温度28°C和pH 9-9.5的条件下，通过34％BuOH液-液萃取（LLE）78分钟（搅拌时间） ）等于264±9.85μgmL -1，这是对其提取的最有利组合。与未优化的提取工艺（219.63±2.48μgmL -1）相比，优化的条件将提取的收率提高了20.20％，同时分别节省了67％（162分钟）和16％的时间和溶剂使用量。与传统方法（36.31±1.37μgmL -1）相比，置换发酵液和随后通过LLE优化提取其提取的肽素总量达到了近七倍。因此，可以经济地生产和提取相对大量的二肽，以用于各种未来的实验。

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