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HPLC ANALYSIS OF PHENOLIC COMPOUNDS FROM ARTEMISIA SPECIES

机译:蒿类中酚类化合物的HPLC分析

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The aim of this study was the identification and quantification of biologically active compounds from three Artemisia species, used as remedies in Romanian traditional medicine. A new LC-MS method was developed for the analysis of methoxylated flavones and also the quantification of caffeic and chlorogenic acids was performed. The antioxidant activity of plant extracts was evaluated by DPPH radical scavenging assay. The paper reports for the first time the presence of eupatorin and hispidulin in A. absinthium and A. vulgaris , of eupatilin in A. annua and casticin in A. vulgaris . Jaceosidin and acacetin were not identified in any sample. Although all extracts showed antiradical scavenging activity, A. vulgaris exhibited the strongest antioxidant effect. Chlorogenic acid was found in high amounts in all species, notably in A. annua and A. vulgaris .
机译:这项研究的目的是鉴定和量化来自三种蒿属物种的生物活性化合物,这些化合物被用作罗马尼亚传统医学的药物。开发了一种新的LC-MS方法用于分析甲氧基黄酮,并进行了咖啡酸和绿原酸的定量分析。通过DPPH自由基清除试验评估植物提取物的抗氧化活性。该文件首次报道了苦艾和寻常曲霉中的eupatorin和hispidulin的出现,寻常的a。annua中的eupatilin和寻常的曲霉中的Casticin的存在。在任何样品中均未鉴定出醋西丁和阿沙西汀。尽管所有提取物均具有抗自由基清除活性,但寻常农杆菌显示出最强的抗氧化作用。在所有物种中均发现大量绿原酸,尤其是在青蒿和寻常农杆菌中。

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