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首页> 外文期刊>European Journal of Histochemistry >Complementary approaches demonstrate that cellular aromatization in the bank vole testis is related to photoperiod
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Complementary approaches demonstrate that cellular aromatization in the bank vole testis is related to photoperiod

机译：补充方法表明，田鼠睾丸中的细胞芳香化与光周期有关

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摘要

A growing body of evidence indicates that germ cells, at least in several mammalian species, are responsible for estrogen formation since they possess active aromatase. In seasonally breeding rodent, the bank vole, the length of photoperiod seems to be the primary environmental factor regulating annual changes in the reproductive activity. However, in this species gonadal steroidogenesis is still not well understood, neither the site of aromatization in testicular cells. In the bank vole testis, aromatase visualized by immunohistochemistry was found in Leydig cells, Sertoli cells, and germ cells: especially in spermatocytes and spermatids. Moreover, in the immuno-electron microscopic study, gold particles indicating aromatase were observed over the cytoplasm of elongated spermatids. The presence of aromatase and the activity of this enzyme were found in microsomal preparations of the whole testes and those of seminiferous tubules. This was measured by means of Western blot and the biochemical assay with tritiated androstenedione, respectively. Additionally, using radioimmunological assays testosterone and estradiol concentrations in homogenates were detected. All the studied parameters revealed close correlation with the length of photoperiod being evidently higher in animals kept in the long day conditions when compared with those from short light cycles.
机译：越来越多的证据表明，至少在几种哺乳动物中，生殖细胞负责雌激素的形成，因为它们具有活性芳香酶。在季节性繁殖的啮齿动物（田鼠）中，光周期的长度似乎是调节生殖活动年度变化的主要环境因素。然而，在该物种中，性腺类固醇的生成尚不十分清楚，睾丸细胞中的芳香化位点也没有。在田鼠的睾丸中，在Leydig细胞，Sertoli细胞和生殖细胞中发现了通过免疫组织化学可视化的芳香酶，尤其是在精细胞和精子细胞中。此外，在免疫电子显微镜研究中，在细长的精子细胞质上观察到表明芳香化酶的金颗粒。在整个睾丸的微粒体制剂和生精小管的微粒体制剂中都发现了芳香化酶的存在和该酶的活性。分别通过蛋白质印迹和and化雄烯二酮的生化测定来测量。另外，使用放射免疫测定法检测匀浆中的睾丸激素和雌二醇浓度。所有研究的参数均显示与长时间光照条件下饲养的动物相比，光周期长度密切相关，而光周期的长度明显高于短光照周期的动物。

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