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首页> 外文期刊>European Journal of Histochemistry >Immunohistochemical detection of C-100 hepatitis C virus antigen in formaldehyde-fixed paraffin-embedded liver tissue. Correlation with serum, tissue and in situ RT-PCR results
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Immunohistochemical detection of C-100 hepatitis C virus antigen in formaldehyde-fixed paraffin-embedded liver tissue. Correlation with serum, tissue and in situ RT-PCR results

机译：甲醛固定石蜡包埋的肝组织中C-100丙型肝炎病毒抗原的免疫组织化学检测。与血清，组织和原位RT-PCR结果的相关性

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摘要

We localized HCV C-100 protein in liver biopsies of 15 patients with chronic hepatitis C using immunohistochemistry. The results were compared to serum, tissue extract analysis of HCV RNA and in situ RT-PCR described in a previous study. HCV was detected in 80% of the sera tested, in 40% of the tissue extracts and in 80% and 60% of the tissue sections tested by immunohistochemistry and in situ RTPCR respectively. Compared to the serum positive cases, 83% and 67% of the cases were respectively positive with immunohistochemistry and in situ RT-PCR and 41% were positive with tissue extract detection. Compared to the tissue extract positive cases, 25% and 50% of the cases were respectively positive with immunohistochemistry and in situ RT-PCR. Finally, 75% of the cases positive by immunohistochemistry were also positive by in situ RT-PCR. These results underline the complementarity of the different methods for the precise diagnosis of hepatitis C.
机译：我们使用免疫组织化学方法在15例慢性丙型肝炎患者的肝活检中定位了HCV C-100蛋白。将结果与先前研究中描述的血清，HCV RNA的组织提取物分析和原位RT-PCR进行了比较。通过免疫组织化学和原位RTPCR分别在80％的血清，40％的组织提取物和80％和60％的组织切片中检测到HCV。与血清阳性病例相比，免疫组织化学和原位RT-PCR阳性病例分别为83％和67％，组织提取物检测阳性病例为41％。与组织提取物阳性病例相比，免疫组织化学和原位RT-PCR分别阳性率为25％和50％。最后，通过免疫组织化学阳性的病例中有75％也通过原位RT-PCR阳性。这些结果强调了精确诊断丙型肝炎的不同方法的互补性。

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