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首页> 外文期刊>European Journal of Medicinal Plants >Premna odorata Volatile Oil as a New Mycobacterium tuberculosis Growth Inhibitor for the Control of Tuberculosis Disease
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Premna odorata Volatile Oil as a New Mycobacterium tuberculosis Growth Inhibitor for the Control of Tuberculosis Disease

机译：Premna odorata挥发油作为控制结核病的新型结核分枝杆菌生长抑制剂

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Aims: This study aimed to identify and compare Premna odorata Blanco volatile oil (VO) for the first time; isolated from different plant organs (leaves, young stems, and flowers) with evaluating the oil antituberculosis (anti-TB) activity. Study Design: Experimental design was carried out by using hydrodistillation method, GC/MS analysis and MeDipro Mycobacterium tuberculosis (MTB) Antigen ELISA Technique (MMA-ELISA) accompanied by polymerase chain reaction (PCR) analysis. Place and Duration of Study: This study was carried out at Faculty of Pharmacy, Beni-Suef University, between May to July 2017. Methodology: P. odorata VO was identified using GC/MS analysis, the oil anti-TB activity was evaluated using in vitro and in vivo MMA-ELISA accompanied by PCR analysis. Results: GC/MS analysis revealed that P. odorata VO consisted of monoterpenes, sesquiterpenes, diterpenes and higher alkanes; where monoterpenes and sesquiterpenes were represented the major oils fractions. Trans-caryophyllene (29.403% & 14.638%) and β-phellandrene (22.390% & 11.701%) were the major compounds in the leaves and young stems oils, respectively. While α-pinene (38.160%) was a characteristic component of the flower's oil. MMA-ELISA showed that a dose of 100 μl/ml in vitro and 300 μl/ml in vivo ; the leaves, young stems, and the flowers oils separately had significant anti-TB activities with measured values > 1.5 μg/ml MTB antigen; while the three organs oils in combination 1:1:1 increased the potency of the oils against MTB with measured values < 1.5 μg/ml MTB antigen with PCR negative analysis. Conclusion: P. odorata VO exhibited Anti-TB activity which Anti-TB could be related to the presence of cyclic terpenes (major) and acyclic oxygenated terpenes (minor) compounds.
机译：目的：本研究旨在首次鉴定和比较香茅（Premna odorata Blanco挥发油）。从不同的植物器官（叶子，幼茎和花朵）中分离出来，并评估其抗油性结核病的活性。研究设计：采用加氢蒸馏法，GC / MS分析和MeDipro结核分枝杆菌（MTB）抗原ELISA技术（MMA-ELISA）进行聚合酶链反应（PCR）分析，进行实验设计。研究的地点和持续时间：该研究于2017年5月至2017年7月在贝尼-苏埃夫大学药学院进行。体外和体内MMA-ELISA结合PCR分析。结果：GC / MS分析表明，香雪松VO由单萜，倍半萜，二萜和高级烷烃组成。其中单萜和倍半萜代表主要的油馏分。反式石竹烯（29.403％＆14.638％）和β-水芹烯（22.390％＆11.701％）分别是叶片油和幼茎油中的主要化合物。而α-pine烯（38.160％）是花朵油的特征成分。 MMA-ELISA显示体外剂量为100μl/ ml，体内剂量为300μl/ ml;叶子，幼茎和花油分别具有明显的抗结核病活性，测量值> 1.5μg/ ml MTB抗原;通过PCR阴性分析，测量值<1.5μg/ ml的MTB抗原，三种器官油以1：1：1的组合增加了油对MTB的效力。结论：香茅VO具有抗TB活性，抗TB可能与环状萜烯（主要）和非环状氧化萜烯（次要）的存在有关。

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