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首页> 外文期刊>European Journal of Histochemistry >Identification of two new repetitive elements and chromosomal mapping of repetitive DNA sequences in the fish Gymnothorax unicolor (Anguilliformes: Muraenidae)
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Identification of two new repetitive elements and chromosomal mapping of repetitive DNA sequences in the fish Gymnothorax unicolor (Anguilliformes: Muraenidae)

机译：鱼类单色Gym中两个新的重复元件的鉴定和重复DNA序列的染色体作图（Anguilliformes：Muraenidae）

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摘要

Muraenidae is a species-rich family, with relationships among genera and species and taxonomy that have not been completely clarified. Few cytogenetic studies have been conducted on this family, and all of them showed the same diploid chromosome number (2n=42) but with conspicuous karyotypic variation among species. The Mediterranean moray eel Gymnothorax unicolor was previously cytogenetically studied using classical techniques that allowed the characterization of its karyotype structure and the constitutive heterochromatin and argyrophilic nucleolar organizer regions (Ag-NORs) distribution pattern. In the present study, we describe two new repetitive elements (called GuMbo I and GuDde I) obtained from restricted genomic DNA of G. unicolor that were characterized by Southern blot and physically localized by in situ hybridization on metaphase chromosomes. As they are highly repetitive DNA sequences, they map in heterochromatic regions. However, while GuDde I was localized in the centromeric regions, the GuMbo I fraction was distributed on some centromeres and was co-localized with the nucleolus organizer region (NOR). Comparative analysis with other Mediterranean species such as Muraena helena pointed out that these DNA fractions are species-specific and could potentially be used for species discrimination. As a new contribution to the karyotype of this species, we found that the major ribosomal genes are localized on acrocentric chromosome 9 and that the telomeres of each chromosome are composed of a tandem repeat derived from a poly-TTAGGG DNA sequence, as it occurs in most vertebrate species. The results obtained add new information useful in comparative genomics at the chromosomal level and contribute to the cytogenetic knowledge regarding this fish family, which has not been extensively studied.
机译：Muraenidae是一个物种丰富的科，其属，种与分类之间的关系尚未完全阐明。对该家族进行的细胞遗传学研究很少，并且所有物种均显示相同的二倍体染色体数（2n = 42），但物种间存在明显的核型变异。以前使用经典技术对地中海海鳗Gym（Gymnothorax unicolor）进行了细胞遗传学研究，该技术可以表征其核型结构以及组成型异染色质和嗜银核仁组织区（Ag-NORs）的分布模式。在本研究中，我们描述了两个新的重复元素（称为GuMbo I和GuDde I），这些元素是从G. unicolor的受限基因组DNA中获得的，其特征在于Southern杂交并通过中期染色体上的原位杂交而物理定位。由于它们是高度重复的DNA序列，因此它们位于异色区域。但是，尽管GuDde I位于着丝粒区域，但GuMbo I组分却分布在某些着丝粒上，并与核仁组织区（NOR）共定位。与其他地中海物种（例如Muraena helena）的比较分析指出，这些DNA片段是特定于物种的，有可能用于物种区分。作为对该物种核型的新贡献，我们发现主要的核糖体基因位于9号近端染色体上，并且每个染色体的端粒均由源自多TTAGGG DNA序列的串联重复序列组成，大多数脊椎动物。获得的结果增加了在染色体水平上可用于比较基因组学的新信息，并有助于对该鱼类家族的细胞遗传学知识的研究，尚未对此进行广泛研究。

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