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Adult stem cells - Biology and methods of analysis

机译:成体干细胞-生物学和分析方法

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I do not like the use of the term adult referred to the somatic stem cells but it is now-a-days impossible to call the cells we are speaking of somatic, it sounds like an incongruous aristocratic attitude, since both laypeople and scientists know very well that we are speaking of those cells able to self renew and to maintain our body efficiently working. Nowadays we find out adult stem cells in every organ we look at and the present day efforts in this area are mainly devoted to work it out new methods to validate the true stemness nature of the cell line we are able to establish. Thus, the three parts that makes up this volume, the first dealing with the basic biology, the second with the characterization of the adult stem cells phenotype and the third with the regulation of the life span of these cells, are all targeted to the critical evaluation of the techniques we use to distinguish adult stem cell-renewal from cell survival. These techniques are mainly focused on the quantification of the replicative life span, the adult stem cell capacity to grow at clonal density, to express pluripotency-related genes .....
机译:我不喜欢使用成人这个词来指代体干细胞,但是如今我们无法称呼我们所说的体细胞,这听起来像一种贵族式的态度,因为外行人和科学家都非常了解好吧,我们所说的是那些能够自我更新并保持人体有效工作的细胞。如今,我们发现了我们所观察的每个器官中的成体干细胞,而如今在这一领域的努力主要致力于寻找新方法来验证我们能够建立的细胞系的真正干性。因此,构成这一体积的三个部分,第一个涉及基础生物学,第二个涉及成体干细胞表型的表征,第三个涉及这些细胞寿命的调节,都针对关键我们用来区分成年干细胞更新与细胞存活的技术的评估。这些技术主要集中在复制寿命的量化,成年干细胞在克隆密度下生长,表达多能性相关基因的能力.....

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