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首页> 外文期刊>European review for medical and pharmacological sciences. >Effects of glutamine-supplemented enteral or parenteral nutrition on apoptosis of intestinal mucosal cells in rats with severe acute pancreatitis
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Effects of glutamine-supplemented enteral or parenteral nutrition on apoptosis of intestinal mucosal cells in rats with severe acute pancreatitis

机译：补充谷氨酰胺的肠内或肠外营养对重症急性胰腺炎大鼠肠黏膜细胞凋亡的影响

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BACKGROUND: Hhigh mortality of the severe acute pancreatitis (SAP) is caused by the damage of intestinal mucosal barrier. Glutamine (Gln) has been used to protect the intestinal mucosal barrier in the treatments of many severe diseases. AIM: To explore the impact of glutamine on the apoptosis of intestinal mucosa and Bax expression in rats with SAP. MATERIALS AND METHODS: Eighty male SD rats were randomly divided into five groups: sham-operated group, SAP + parenteral nutrition (PN) group, SAP + enteral nutrition (EN) group, SAP + EN + Gln group, and SAP + PN + Gln group. Rats were sacrificed 4 days and 7 days after nutritional support. The intestinal epithelial apoptosis and the expression of Bax were examined by TUNEL assay and immunohistochemical staining, respectively. RESULTS: The apoptotic index (AI) of SAP groups was higher than that of sham-operated group. After 7 days treatment, the AI of SAP + EN + Gln group was significantly lower than that of the SAP + EN or SAP + PN group. In addition, the AI of the SAP groups after 7 days treatment was significantly lower than that of the same groups at 4 days after treatment. Furthermore, the Bax expression of SAP + EN + Gln group was significantly lower than that in the SAP + EN or SAP + PN group. However, no significant differences were observed between SAP + EN + Gln group and SAP + PN + Gln group in AI and Bax expression. CONCLUSIONS: Combination of Gln and parenteral nutrition or enteral nutrition inhibits the apoptosis of intestinal epithelial cells and maintains the integrity of the intestinal mucosal barrier.
机译：背景：严重急性胰腺炎（SAP）的高死亡率是由肠粘膜屏障的破坏引起的。谷氨酰胺（Gln）在许多严重疾病的治疗中已被用来保护肠粘膜屏障。目的：探讨谷氨酰胺对SAP大鼠肠黏膜凋亡及Bax表达的影响。材料与方法：80只雄性SD大鼠随机分为5组：假手术组，SAP +肠外营养（PN）组，SAP +肠内营养（EN）组，SAP + EN + Gln组，SAP + PN + Gln组。营养支持后第4天和第7天处死大鼠。分别通过TUNEL法和免疫组织化学染色检查肠上皮细胞凋亡和Bax的表达。结果：SAP组的凋亡指数（AI）高于假手术组。治疗7天后，SAP + EN + Gln组的AI显着低于SAP + EN或SAP + PN组的AI。另外，治疗7天后SAP组的AI显着低于治疗后4天相同组的AI。此外，SAP + EN + Gln组的Bax表达明显低于SAP + EN或SAP + PN组。但是，SAP + EN + Gln组和SAP + PN + Gln组之间在AI和Bax表达上没有观察到显着差异。结论：Gln与肠胃外营养或肠内营养的结合可抑制肠上皮细胞的凋亡，并维持肠粘膜屏障的完整性。

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