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首页> 外文期刊>European review for medical and pharmacological sciences. >Influences of remifentanil on myocardial ischemia-reperfusion injury and the expressions of Bax and Bcl-2 in rats
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Influences of remifentanil on myocardial ischemia-reperfusion injury and the expressions of Bax and Bcl-2 in rats

机译：瑞芬太尼对大鼠心肌缺血再灌注损伤及Bax和Bcl-2表达的影响

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OBJECTIVE: To investigate the influences of remifentanil on myocardial ischemia-reperfusion injury in rats and the expressions of b-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax) and other apoptosis-related proteins. MATERIALS AND METHODS: A total of 60 Sprague-Dawley (SD) rats were randomly divided into sham operation (S) group, model (M) group, low-dose remifentanil (L) group and high-dose remifentanil (H) group, with 15 rats in each group. The rat model of myocardial ischemia-reperfusion injury was established by the ligation of the left anterior descending branch (LAD). After ischemia for 30 min and reperfusion for 24 h, the cardiac function of rats in each group was measured by an ultrasonic instrument. Triphenyl tetrazolium chloride (TTC) staining was used to detect the myocardial infarction area of rats in each group. The activity of myocardial enzymes in the serum of rats in each group was detected. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was adopted to examine the apoptosis level of rat cardiomyocytes in each group. Quantitative polymerase chain reaction (qPCR) and Western blotting were applied to detect the expression levels of apoptosis-related proteins and messenger ribonucleic acids (mRNAs) in rat cardiomyocytes in each group. RESULTS: Compared with those in S group, left ventricular internal dimension in systole (LVIDs) and left ventricular internal dimension in diastole (LVIDd) were markedly increased (p0.01), while left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) were notably decreased in M group (p0.01). LVIDs and LVIDd in L group and H group were lower than those in M group (p0.05, p0.01), whereas LVEF and LVFS were higher than those in M group (p0.05, p0.01). The myocardial infarction area in M group was significantly larger than that in S group (p0.01), and those in L group and H group were remarkably smaller than that in M group (p0.05, p0.01). The activities of aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase-muscle/brain (CK-MB) in the serum of rats in M group were evidently higher than those in S group (p0.01), and compared with those in M group, those in L group and H group were significantly decreased (p0.05, p0.01). The apoptosis level of myocardial cells in M group was significantly higher than that in S group (p0.01), while those in L group and H group were markedly lower than that in M group (p0.05, p0.01). Compared with those in S group, the expression levels of cleaved caspase-3 and its mRNAs in myocardial cells in M group were remarkably increased (p0.01), while those of Bcl-2/Bax and it mRNAs were significantly decreased (p0.01). The expression levels of cleaved caspase-3 and its mRNAs in myocardial cells in L group and H group were significantly lower than those in M group (p0.05, p0.01), but those of Bcl-2/Bax and its mRNAs were significantly higher than those in M group (p0.05, p0.01). CONCLUSIONS: Remifentanil can effectively reduce myocardial cell injury caused by myocardial ischemia-reperfusion in rats, improve cardiac function, reduce the myocardial infarction area, decrease cleaved caspase-3 in myocardial cells, and increase Bcl-2/Bax.
机译：目的：观察瑞芬太尼对大鼠心肌缺血再灌注损伤的影响以及b细胞淋巴瘤2（Bcl-2），Bcl-2相关X蛋白（Bax）及其他凋亡相关蛋白的表达。材料与方法：60只SD大鼠随机分为假手术组，模型组，低剂量瑞芬太尼组和高剂量瑞芬太尼组。每组有15只大鼠。结扎左前降支（LAD）建立大鼠心肌缺血再灌注损伤模型。缺血30分钟并再灌注24小时后，用超声仪测量每组大鼠的心功能。三苯基氯化四唑（TTC）染色用于检测每组大鼠的心肌梗塞面积。检测各组大鼠血清中心肌酶的活性。采用末端脱氧核苷酸转移酶dUTP缺口末端标记法（TUNEL）染色观察各组大鼠心肌细胞的凋亡水平。定量聚合酶链反应（qPCR）和蛋白质印迹法检测每组大鼠心肌细胞中凋亡相关蛋白和信使核糖核酸（mRNA）的表达水平。结果：与S组比较，收缩期左室内部尺寸（LVIDs）和舒张期左室内部尺寸（LVIDd）明显增加（p <0.01），而左室射血分数（LVEF）和左室分数缩短M组（LVFS）明显降低（p <0.01）。 L组和H组的LVIDs和LVIDd低于M组（p <0.05，p <0.01），而LVEF和LVFS高于M组（p <0.05，p <0.01）。 M组的心肌梗死面积明显大于S组（p <0.01），L组和H组的心肌梗塞面积明显小于M组（p <0.05，p <0.01）。 M组大鼠血清中的天冬氨酸转氨酶（AST），乳酸脱氢酶（LDH）和肌酸激酶-肌肉/脑（CK-MB）活性明显高于S组（p <0.01），并进行了比较与M组相比，L组和H组明显降低（p <0.05，p <0.01）。 M组心肌细胞的凋亡水平明显高于S组（p <0.01），而L组和H组的心肌细胞凋亡水平明显低于M组（p <0.05，p <0.01）。与S组相比，M组心肌细胞中裂解的caspase-3及其mRNA的表达水平明显升高（p <0.01），而Bcl-2 / Bax及其mRNA的表达明显降低（p <0.01）。 0.01）。 L组和H组心肌细胞裂解的caspase-3及其mRNA的表达水平明显低于M组（p <0.05，p <0.01），而Bcl-2 / Bax及其mRNA的表达水平明显低于M组（p <0.05，p <0.01）。显着高于M组（p <0.05，p <0.01）。结论：瑞芬太尼可有效减轻大鼠心肌缺血/再灌注引起的心肌细胞损伤，改善心脏功能，减少心肌梗塞面积，减少心肌细胞中caspase-3的裂解，并增加Bcl-2 / Bax。

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