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β hydroxybutyrate levels in serum and cerebrospinal fluid under ketone body metabolism in rats

机译：酮体代谢对大鼠血清和脑脊液中β-羟基丁酸水平的影响

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A high-fat, low–carbohydrate diet (KD) or calorie restriction in the form of every-other-day fasting (EODF) results in ketone body metabolism with an increasing β-hydroxybutyrate (βOHB) level. Previous studies have supported that a KD and EODF have a neuroprotective effect. However, the βOHB levels in the cerebrospinal fluid (CSF) resulting from a KD and EODF remain unknown. The aim of this study was to detect βOHB levels in rats fed a KD, EODF diet, and every-other-day ketogenic diet (EODKD) and to compare the serum βOHB level with the CSF βOHB level. Twenty-four male Sprague-Dawley rats were randomly divided into KD, EODF, EODKD, and standard diet (SD) groups. A customized food with a ratio of carbohydrates to fats of 1:4 was used in the KD and EODKD groups. The βOHB level was measured using ELISA kits in 200 μ l serum and 100 μ l CSF samples for each rat after feeding for 2 weeks. The KD, EODF, and EODKD resulted in a significant increase in βOHB levels in both the serum and CSF. The βOHB levels in the EODKD group were the highest. The CSF βOHB level was, on average, 69% of the serum βOHB level. There was a positive correlation between the overall βOHB levels in serum and that in cerebrospinal fluid. This study demonstrated that the KD, EODF, and EODKD resulted in ketone body metabolism, as the βOHB levels increased significantly compared with those resulting from the standard diet. Our results suggested that the serum βOHB level was an indicator of the CSF βOHB level, and that the EODKD was an effective diet to enhance ketogenic metabolism.
机译：高脂肪，低碳水化合物饮食（KD）或每隔一天禁食（EODF）形式的卡路里限制会导致酮体代谢，并增加β-羟基丁酸酯（βOHB）含量。先前的研究支持KD和EODF具有神经保护作用。然而，由KD和EODF引起的脑脊液（CSF）中的βOHB水平仍然未知。这项研究的目的是检测饲喂KD，EODF饮食和隔日生酮饮食（EODKD）的大鼠中的βOHB水平，并将血清βOHB水平与CSFβOHB水平进行比较。将24只雄性Sprague-Dawley大鼠随机分为KD，EODF，EODKD和标准饮食（SD）组。在KD和EODKD组中使用了碳水化合物与脂肪之比为1：4的定制食物。喂食2周后，使用ELISA试剂盒在每只大鼠200μl血清和100μlCSF样品中测量βOHB水平。 KD，EODF和EODKD导致血清和CSF中βOHB含量显着增加。 EODKD组中的βOHB水平最高。 CSFβOHB水平平均为血清βOHB水平的69％。血清中总βOHB水平与脑脊液中βOHB水平呈正相关。这项研究表明，KD，EODF和EODKD会导致酮体代谢，因为与标准饮食相比，βOHB含量显着增加。我们的结果表明，血清βOHB水平是CSFβOHB水平的指标，而EODKD是增强生酮代谢的有效饮食。

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