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首页> 外文期刊>Experimental Animals >Preventive effect of intravesical ozone supplementation on n-methyl-n-nitrosourea-induced non-muscle invasive bladder cancer in male rats
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Preventive effect of intravesical ozone supplementation on n-methyl-n-nitrosourea-induced non-muscle invasive bladder cancer in male rats

机译：膀胱内补充臭氧对n-甲基-n-亚硝基脲诱导的雄性大鼠非肌肉浸润性膀胱癌的预防作用

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Although non-muscle invasive bladder cancer (NMIBC) is widely seen in men, most laboratory studies of new intravesical therapies to prevent NMIBC have been conducted on female animals. In addition, ozone (O3) has been shown to be a beneficial agent as an intravesical application in the treatment of various disorders. In the current study, we evaluated the immunohistopathological and oxidative-antioxidative effects of intravesical O3 treatment on n -methyl- n -nitrosourea (MNU)-induced NMIBC. Male Wistar-Albino rats (n=51) were divided into four groups: sham (n=6), O3 only (n=15), MNU only (n=15), and MNU+O3 (n=15). The MNU-only and MNU+O3 groups received MNU, and the O3-only group received saline every other week for 10 weeks. The MNU-only group received 1 ml saline in place of O3 treatment, whereas the O3-only and MNU+O3 groups were treated with 1 ml 25 μ g/ml O3 between the 7th and 12th weeks. Rat bladders were collected in the 15th week for immunohistopathology and oxidant-antioxidant quantitation. Oxidant-antioxidant parameters were determined by ELISA. Although all surviving rats in the MNU-only group had preneoplastic (4/11, 36.4%) or neoplastic changes (7/11, 63.6%), a completely normal urothelium was observed in 2 rats (2/12, 16.7%) in the MNU+O3-group ( P =0.478). More high-grade lesions were observed in the MNU-only group (4/11, 36.4%) than in the MNU+O3 group (1/12, 8.3%) ( P =0.120). All oxidant-antioxidant parameters significantly increased ( P 3-only group compared with the sham group. However, only antioxidant superoxide dismutase was remarkably higher (178.9%, P =0.060) in the MNU+O3 group compared with the MNU-only group. This is the first methodologically and pathologically well-described male rat orthotopic bladder carcinogenesis model with intravesical MNU and administration of O3 in NMIBC.
机译：尽管非肌肉浸润性膀胱癌（NMIBC）在男性中很普遍，但是大多数预防新的膀胱内治疗以预防NMIBC的实验室研究都是在雌性动物身上进行的。此外，臭氧（O 3 ）已被证明是膀胱内治疗多种疾病的有益药物。在本研究中，我们评估了膀胱内O 3 处理对n-甲基-n-亚硝基脲（MNU）诱导的NMIBC的免疫组织病理学和氧化-抗氧化作用。 Wistar-Albino雄性大鼠（n = 51）分为四组：假（n = 6），仅O 3 （n = 15），仅MNU（n = 15）和MNU + O 3 （n = 15）。仅MNU和MNU + O 3 组接受MNU，仅O 3 组每隔一周接受生理盐水治疗10周。仅MNU组接受1 ml盐水代替O 3 处理，而仅O 3 和MNU + O 3 组为在第7周和第12周之间用1 ml 25μg / ml O 3 处理。在第15周收集大鼠膀胱用于免疫组织病理学和氧化剂-抗氧化剂定量。通过ELISA确定氧化剂-抗氧化剂参数。尽管仅MNU组中所有存活的大鼠均出现肿瘤前改变（4/11，36.4％）或肿瘤改变（7/11，63.6％），但在2只大鼠中观察到完全正常的尿道上皮（2/12，16.7％）。 MNU + O 3 -组（P = 0.478）。仅MNU组（4 / 11，36.4％）比MNU + O 3 组（1 / 12，8.3％）观察到更高级别的病变（P = 0.120）。与假手术组相比，所有抗氧化剂参数均显着增加（仅P 3 组），但在MNU + O 3 <中，仅抗氧化剂超氧化物歧化酶显着较高（178.9％，P = 0.060）。 / sub>组与仅MNU组相比，这是第一个在方法学和病理学上被描述良好的雄性大鼠原位膀胱癌发生模型，其中包括膀胱内MNU和在NMIBC中施用O 3 。

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