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首页> 外文期刊>Eukaryotic cell >Developmental Growth Control Exerted via the Protein A Kinase Tpk2 in Ashbya gossypii
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Developmental Growth Control Exerted via the Protein A Kinase Tpk2 in Ashbya gossypii

机译：通过Ashbya棉酚中的蛋白A激酶Tpk2进行发育生长控制。

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Sporulation in Ashbya gossypii is induced by nutrient-limited conditions and leads to the formation of haploid spores. Using RNA-seq, we have determined a gene set induced upon sporulation, which bears considerable overlap with that of Saccharomyces cerevisiae but also contains A. gossypii-specific genes. Addition of cyclic AMP (cAMP) to nutrient-limited media blocks sporulation and represses the induction of sporulation specific genes. Deletion of the protein kinase A (PKA) catalytic subunits encoded by TPK1 and TPK2 showed reduced growth in tpk1 but enhanced growth in the tpk2 strain; however, both mutants sporulated well. Sporulation can be blocked by cAMP in tpk1 but not in tpk2 strains. Similarly, TPK2 acts at a second developmental switch promoting the break in spore dormancy. In S. cerevisiae, PKA phosphorylates and inhibits Msn2/4. The transcript profiles of the tpk1 and msn2/4 mutants were very similar to that of the wild type under sporulation conditions. However, deletion of the single A. gossypii MSN2/4 homolog generated a specific sporulation defect. We identified a set of genes involved in spore wall assembly that was downregulated in the msn2/4 mutant, particularly DIT2, suggesting that poor spore viability may be due to lysis of spores. Our results reveal specific functional differences between the two catalytic PKA subunits in A. gossypii and identified Tpk2 as the key A kinase that transduces developmental decisions of growth. Our data also suggest that Msn2/4 is involved only at a late step of sporulation in A. gossypii and is not a major regulator of IME1.
机译：在营养有限的条件下诱导棉球菌的孢子形成并导致单倍体孢子的形成。使用RNA-seq，我们确定了在芽孢形成后诱导的基因集，该基因集与酿酒酵母有相当大的重叠，但也含有棉孢曲霉特异的基因。在营养有限的培养基中添加环状AMP（cAMP）可阻止孢子形成并抑制孢子形成特异性基因的诱导。 TPK1 和 TPK2 编码的蛋白激酶A（PKA）催化亚基的删除显示 tpk1 的生长减少，但的生长增强tpk2 株;但是，两个突变体的孢子形成都很好。 cAMP在 tpk1 中可以阻止孢子形成，而在 tpk2 菌株中则不能。同样， TPK2 在第二次发育转换中起作用，促进了孢子休眠的中断。在酿酒酵母中，PKA磷酸化并抑制Msn2 / 4。在芽孢形成条件下， tpk1 和 msn2 / 4 突变体的转录本特征与野生型非常相似。然而，单个棉曲霉 MSN2 / 4 同源物的缺失产生了特定的孢子形成缺陷。我们发现了一组与孢子壁组装有关的基因，这些基因在 msn2 / 4 突变体（尤其是 DIT2 ）中被下调，这表明孢子活力差可能是由于孢子的裂解。我们的结果揭示了棉铃虫中两个催化性PKA亚基之间的特定功能差异，并将Tpk2确定为转导生长发育决定的关键A激酶。我们的数据还表明，Msn2 / 4仅在棉孢曲霉孢子形成的后期才参与，而不是 IME1 的主要调节因子。

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