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首页> 外文期刊>Eukaryotic cell >Molecular Studies Reveal Frequent Misidentification of Aspergillus fumigatus by Morphotyping
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Molecular Studies Reveal Frequent Misidentification of Aspergillus fumigatus by Morphotyping

机译：分子研究揭示了通过形态分型法对烟曲霉的常见错误鉴定

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Aspergillus fumigatus has been understood to be the most common cause of invasive aspergillosis (IA) in all epidemiological surveys. However, recent studies have uncovered a large degree of genetic heterogeneity between isolates morphologically identified as A. fumigatus, leading to the description of a new species, Aspergillus lentulus. Here, we examined the genetic diversity of clinical isolates identified as A. fumigatus using restriction enzyme polymorphism analysis and sequence-based identification. Analysis of 50 clinical isolates from geographically diverse locations recorded the presence of at least three distinct species: A. lentulus, Aspergillus udagawae, and A. fumigatus. In vitro, A. lentulus isolates demonstrated decreased susceptibility to antifungal drugs currently used for IA, including amphotericin B, voriconazole, and caspofungin; A. udagawae isolates demonstrated decreased in vitro susceptibility to amphotericin B. Results of the present study demonstrate that current phenotypic methods to identify fungi do not differentiate between genetically distinct species in the A. fumigatus group. Differential antifungal susceptibilities of these species may account for some of the reported poor outcomes of therapy in clinical studies.
机译：在所有流行病学调查中，烟曲霉被认为是侵袭性曲霉病（IA）的最常见原因。然而，最近的研究发现在形态学上被鉴定为 A的分离株之间存在很大程度的遗传异质性。烟，导致描述了一种新物种，即 lentilus lentulus 。在这里，我们检查了鉴定为 A的临床分离株的遗传多样性。限制酶多态性分析和基于序列的鉴定方法对烟对来自不同地理位置的50种临床分离株的分析记录了至少三种不同物种的存在： A。扁豆，乌达氏曲霉和 A。烟熏。在体外， A。小扁豆菌株对目前用于IA的抗真菌药物包括两性霉素B，伏立康唑和卡泊芬净的敏感性降低。 A。 udagawae 分离株显示出对两性霉素B的体外敏感性降低。本研究结果表明，目前鉴定真菌的表型方法不能区分 A中遗传上不同的物种。烟熏组。在临床研究中，这些物种的抗真菌药敏性差异可能是一些治疗效果差的原因。

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