...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Eukaryotic cell >Changes in the N-Glycome, Glycoproteins with Asn-Linked Glycans, of Giardia lamblia with Differentiation from Trophozoites to Cysts
【24h】

Changes in the N-Glycome, Glycoproteins with Asn-Linked Glycans, of Giardia lamblia with Differentiation from Trophozoites to Cysts

机译：贾第虫（Giardia lamblia）N糖，糖蛋白与Asn连接的聚糖的变化，从滋养体到囊肿的分化

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Giardia lamblia is present in the intestinal lumen as a binucleate, flagellated trophozoite or a quadranucleate, immotile cyst. Here we used the plant lectin wheat germ agglutinin (WGA), which binds to the disaccharide di-N-acetyl-chitobiose (GlcNAc2), which is the truncated Asn-linked glycan (N-glycan) of Giardia, to affinity purify the N-glycomes (glycoproteins with N-glycans) of trophozoites and cysts. Fluorescent WGA bound to the perinuclear membranes, peripheral acidified vesicles, and plasma membranes of trophozoites. In contrast, WGA bound strongly to membranes adjacent to the wall of Giardia cysts and less strongly to the endoplasmic reticulum and acidified vesicles. WGA lectin-affinity chromatography dramatically enriched secreted and membrane proteins of Giardia, including proteases and acid phosphatases that retain their activities. With mass spectroscopy, we identified 91 glycopeptides with N-glycans and 194 trophozoite-secreted and membrane proteins, including 42 unique proteins. The Giardia oligosaccharyltransferase, which contains a single catalytic subunit, preferred N glycosylation sites with Thr to those with Ser in vivo but had no preference for flanking amino acids. The most-abundant glycoproteins in the N-glycome of trophozoites were lysosomal enzymes, folding-associated proteins, and unique transmembrane proteins with Cys-, Leu-, or Gly-rich repeats. We identified 157 secreted and membrane proteins in the Giardia cysts, including 20 unique proteins. Compared to trophozoites, cysts were enriched in Gly-rich repeat transmembrane proteins, cyst wall proteins, and unique membrane proteins but had relatively fewer Leu-rich repeat proteins, folding-associated proteins, and unique secreted proteins. In summary, there are major changes in the Giardia N-glycome with the differentiation from trophozoites to cysts.
机译：贾第鞭毛虫（Giardia lamblia）在肠腔中以双核，鞭毛状滋养体或四核，不运动性囊肿存在。在这里，我们使用了植物凝集素小麦胚芽凝集素（WGA），该凝集素与二糖di- N -乙酰壳聚糖二糖（GlcNAc 2 ）结合，后者是截短的Asn-连接贾第鞭毛虫的聚糖（N-聚糖），以亲和纯化滋养体和囊肿的 N -糖蛋白（带有N-聚糖的糖蛋白）。荧光WGA结合到滋养体的核周膜，外周酸化囊泡和质膜上。相反，WGA与贾第鞭毛虫囊壁的膜紧密结合，而与内质网和酸化囊泡的结合较弱。 WGA凝集素亲和色谱显着丰富了贾第鞭毛虫的分泌蛋白和膜蛋白，包括可保持其活性的蛋白酶和酸性磷酸酶。通过质谱，我们确定了91种具有N-聚糖的糖肽和194种滋养体分泌的膜蛋白，其中包括42种独特的蛋白。含有单个催化亚基的寡糖基转移酶比体内具有Ser的那些更优选具有Thr的N个糖基化位点，但对侧翼氨基酸没有偏爱。滋养体 N 糖基中最丰富的糖蛋白是溶酶体酶，折叠相关蛋白和富含Cys，Leu或Gly重复序列的独特跨膜蛋白。我们在贾第虫（Giardia）囊肿中鉴定了157种分泌蛋白和膜蛋白，其中包括20种独特的蛋白。与滋养体相比，囊肿富含富含甘氨酸的重复跨膜蛋白，囊壁蛋白和独特的膜蛋白，但富含亮氨酸的重复蛋白，折叠相关蛋白和独特的分泌蛋白相对较少。总之，随着滋养体向囊肿的分化，Giardia N 糖组发生了重大变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号