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首页> 外文期刊>Gene Therapy and Molecular Biology >Mutations of tumour suppressor gene P53 (TP53) intumour tissue and cellular urine sediments inurinary bladder cancer
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Mutations of tumour suppressor gene P53 (TP53) intumour tissue and cellular urine sediments inurinary bladder cancer

机译：膀胱癌中肿瘤抑制基因P53（TP53）在肿瘤组织和细胞尿沉渣中的突变

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TP53 mutations are frequently correlated with tumour development in bladder cancer. One function of TP53 is the suppression of apoptosis. We compared TP53 mutation in tumour tissue and urine sediment of urinary bladder cancer patients. We examined 32 patients with urinary bladder cancer. Screening for TP53 mutations in tumour tissue and urine sediment: Amplification of the TP53 gene by polymerase chain reaction (PCR) for the exons 5, 6, 7 and 8; Temperature gradient gel electrophoresis (TGGE) was used to analyse the TP53 mutations. We detected mutations of TP53 in invasive bladder cancer in 5 of 5 cases (100%); in superficial bladder cancer we found 20 of 27 cases (74%) with mutation of TP53. In 14 of 25 patients (56%) we could find TP53 mutation mobility shifts in the same exons analyzed in both tumour tissue and urine sediment. In some cases we could localize the same mutation of TP53 in both materials. TP53 mutations are detectable in early bladder cancer stages by PCR-TGGE and sequencing, which might influence therapeutic strategies. The identification of gene mutations in extracorporal samples, such as urine sediment, is an important area of research having implications for tumour diagnosis and monitoring. This study show that TP53 mutation are detectable in tumour tissue and in cellular urine sediment; beside that we demonstrate that TP53 mutation frequently occur in higher stages and grades of bladder tumours. The included review of the literature and the results of this study make clear that scientific work in the field of tumour markers in urinary bladder cancer is an important theme in urology nowadays and in future.
机译：TP53突变经常与膀胱癌的肿瘤发展相关。 TP53的功能之一是抑制细胞凋亡。我们比较了膀胱癌患者肿瘤组织和尿沉渣中的TP53突变。我们检查了32例膀胱癌患者。筛选肿瘤组织和尿沉渣中的TP53突变：通过聚合酶链反应（PCR）扩增外显子5、6、7和8的TP53基因;使用温度梯度凝胶电泳（TGGE）分析TP53突变。我们在5例病例中有5例（100％）检测到浸润性膀胱癌中TP53的突变;在浅表性膀胱癌中，我们发现27例中有20例（74％）TP53突变。在25位患者中的14位（56％）中，我们可以在肿瘤组织和尿沉渣中分析的相同外显子中发现TP53突变迁移率变化。在某些情况下，我们可以在两种材料中定位相同的TP53突变。通过PCR-TGGE和测序可以在膀胱癌早期检测到TP53突变，这可能会影响治疗策略。体外样本（如尿沉渣）中基因突变的鉴定是重要的研究领域，对肿瘤的诊断和监测具有重要意义。这项研究表明，在肿瘤组织和细胞尿液沉积物中可检测到TP53突变。除此之外，我们证明了TP53突变经常发生在膀胱肿瘤的更高阶段和等级。所包括的文献综述和本研究的结果表明，在膀胱癌的肿瘤标志物领域的科学工作是当今和将来泌尿外科的重要主题。

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