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首页> 外文期刊>Experimental and clinical transplantation >Human Islet Cell Isolation: The Initial Step in an Islet Transplanting Program in Shiraz, Southern Iran
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Human Islet Cell Isolation: The Initial Step in an Islet Transplanting Program in Shiraz, Southern Iran

机译：人胰岛细胞分离：伊朗南部设拉子的胰岛移植计划的第一步

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Objectives: Type 1 diabetes mellitus is an emerging epidemic worldwide and results from autoimmune destruction of insulin-producing β cells. Islet transplanting is a potential treatment for type 1 diabetes mellitus. Materials and Methods: The Shiraz Organ Transplant Center is a leading center for organ transplants, especially pancreatic transplants, in Iran. For this reason, we want to establish an islet transplanting program. Here, we briefly describe our experience with islet isolation on 6 pancreata from deceased donors. We discussed the necessary equipment required for this procedure, as well as the professionals needed and a specially planned facility. Results: Islet yield was ≤ 100 000 (islet equivalent), viability 40% to 45%, and the purity was 30% to 45%. We do not have a refrigerated COBE processor for purification; therefore, the yield was low. Our experience shows that we should improve things, so as to acquire more islets for developing clinical grade cell therapy. Conclusions: Overall, isolation costs are high, and accessing a safer, more economic, and persistent source of material and reagents will improve this technique.
机译：目的：1型糖尿病是全球范围内的一种新型流行病，其原因是胰岛素产生的β细胞自身免疫破坏。胰岛移植是1型糖尿病的潜在治疗方法。材料和方法：设拉子器官移植中心是伊朗器官移植特别是胰腺移植的主要中心。因此，我们要建立一个胰岛移植程序。在这里，我们简要介绍了我们对死者捐赠的6种胰岛进行胰岛分离的经验。我们讨论了此过程所需的必要设备，以及所需的专业人员和特别计划的设施。结果：胰岛产量≤100,000（胰岛当量），生存力40％至45％，纯度为30％至45％。我们没有用于纯化的冷藏COBE处理器;因此，产率很低。我们的经验表明，我们应该进行改善，以获取更多的胰岛用于开发临床级细胞疗法。结论：总的来说，隔离成本很高，并且获得更安全，更经济和持久的材料和试剂来源将改善该技术。

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