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首页> 外文期刊>Experimental diabetes research >The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9
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The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9

机译：高浓度MMP9可以抑制大鼠皮肤成纤维细胞的生物学行为。

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Aims. To explore the effects of the high expression of MMP9 on biological behaviors of fibroblasts.Methods. High glucose and hyperhomocysteine were used to induce MMP9 expression in skin fibroblasts. Cell proliferation was detected by flow cytometry and cell viability by CCK-8. ELISA assay was used to detect collagen (hydroxyproline) secretion. Scratch test was employed to evaluate horizontal migration of cells and transwell method to evaluate vertical migration of cells.Results. The mRNA and protein expressions of MMP9 and its protease activity were significantly higher in cells treated with high glucose and hyperhomocysteine than those in control group. At the same time, the S-phase cell ratio, proliferation index, cell viability, collagen (hydroxyproline) secretion, horizontal migration rate, and the number of vertical migration cells decreased in high-glucose and hyperhomocysteine-treated group. Tissue inhibitor of metalloproteinase 1 (TIMP1), which inhibits the activity of MMP9, recovered the above biological behaviors.Conclusions. High expression of MMP9 in skin fibroblasts could be induced by cultureing in high glucose and hyperhomocysteine medium, which inhibited cell biological behaviors. Inhibitions could be reversed by TIMP1. The findings suggested that MMP9 deters the healing of diabetic foot ulcers by inhibiting the biological behaviors of fibroblasts.
机译：目的探讨MMP9的高表达对成纤维细胞生物学行为的影响。高葡萄糖和高半胱氨酸被用来诱导皮肤成纤维细胞中MMP9的表达。通过流式细胞术检测细胞增殖，并通过CCK-8检测细胞活力。 ELISA分析用于检测胶原蛋白（羟脯氨酸）的分泌。采用刮擦试验评价细胞水平迁移，采用transwell法评价细胞垂直迁移。高糖和高同型半胱氨酸处理的细胞中MMP9的mRNA和蛋白表达及其蛋白酶活性显着高于对照组。同时，高糖和高同型半胱氨酸治疗组的S期细胞比例，增殖指数，细胞生存力，胶原蛋白（羟脯氨酸）分泌，水平迁移率和垂直迁移细胞数量减少。抑制MMP9活性的金属蛋白酶1组织抑制剂（TIMP1）恢复了上述生物学行为。在高葡萄糖和高同型半胱氨酸培养基中培养可以诱导皮肤成纤维细胞中MMP9的高表达，从而抑制细胞生物学行为。 TIMP1可以逆转抑制作用。这些发现表明，MMP9通过抑制成纤维细胞的生物学行为来阻止糖尿病足溃疡的愈合。

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