A Human Papillomavirus (HPV) - basedpseudoviral gene delivery system for the non-viral,Episomally Replicating Vector pEPI-1

Dr. Armin Baiker

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A Human Papillomavirus (HPV) - basedpseudoviral gene delivery system for the non-viral,Episomally Replicating Vector pEPI-1

机译：基于人乳头瘤病毒（HPV）的伪病毒基因传递系统，用于非病毒，表观复制载体pEPI-1

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Non-viral episomal vector systems might allow a safe and reproducible genetic modification of eukaryotic cells and organisms. However, they share with all non-viral systems the problem of low efficient delivery to the target cells. To overcome this limitation we have utilized a Human Papillomavirus type 16 (HPV16)-based pseudoviral gene delivery system for the non-viral episomally replicating plasmid vector pEPI-1. Pseudoviral particles harbouring pEPI-1 (pEPI-PV) were prepared by co-transfection of the pEPI-1 plasmid DNA and a plasmid expressing the capsid proteins L1 and L2 of HPV16. Here we demonstrate that the resulting pEPI-PV were able to transduce target CHO cells with high efficiency under maintenance of the episomal character of pEPI-1. The combination of HPV-based pseudoviral gene delivery and non-viral, episomally replicating plasmids will provide a powerful new tool for biotechnical and possibly in vivo biomedical applications.

机译：非病毒游离载体系统可能允许对真核细胞和生物体进行安全且可重复的遗传修饰。然而，它们与所有非病毒系统共享向靶细胞的低效率递送的问题。为克服此限制，我们已将基于人乳头瘤病毒16型（HPV16）的假病毒基因传递系统用于非病毒复制型质粒载体pEPI-1。通过共转染pEPI-1质粒DNA和表达HPV16衣壳蛋白L1和L2的质粒，制备了带有pEPI-1（pEPI-PV）的伪病毒颗粒。在这里，我们证明了所得的pEPI-PV能够在维持pEPI-1附加特征的情况下高效转导靶CHO细胞。基于HPV的假病毒基因传递与非病毒，游离复制质粒的结合将为生物技术以及可能的体内生物医学应用提供强大的新工具。

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《Gene Therapy and Molecular Biology》 |2005年第1期|共6页
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Dr. Armin Baiker;
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1. Non-viral S/MAR vectors replicate episomally in vivo when provided with a selective advantage. [J] . Wong SP, Argyros O, Coutelle C, Gene therapy . 2011,第1期

机译：当提供选择性优势时，非病毒S / MAR载体可在体内游离复制。
2. Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector. [J] . Argyros O, Wong SP, Niceta M, Gene therapy . 2008,第24期

机译：在递送包含非病毒载体的支架/基质附着区域后，肝脏中持续的游离型转基因表达。
3. Generation of a tumor- and tissue-specific episomal non-viral vector system [J] . Rudolf Haase, Terese Magnusson, Baowei Su, BMC Biotechnology . 2013,第1期

机译：肿瘤和组织特异性游离型非病毒载体系统的产生
4. Human papillomavirus genetic immunization system based on HPVL1- chemokine fusion [C] . Yu-Kyoung Oh, Sang-eun Park, Jeong Sook Park, Proceedings of the 29th Annual Meeting of the Controlled Release Society . 2002

机译：基于HPVL1-趋化因子融合的人乳头瘤病毒基因免疫系统
5. Biodegradable branched polycationic polymers as non-viral gene delivery vectors for bone tissue engineering. [D] . Chew, Sue Anne. 2010

机译：可生物降解的支链聚阳离子聚合物，作为骨组织工程的非病毒基因递送载体。
6. Long-Term Suppression of Hepatitis B Virus Replication by Short Hairpin RNA Expression Using the Scaffold/Matrix Attachment Region-Based Replicating Vector System pEPI-1 [O] . Andreas C. W. Jenke, Andreas D. Wilhelm, Valerie Orth, 2008

机译：通过使用基于支架/基质附着区的复制载体系统pEPI-1的短发夹RNA表达来长期抑制乙型肝炎病毒的复制
7. Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector [O] . Argyros, O, Wong, SP, Niceta M, 2008

机译：交付包含非病毒载体的支架/基质附着区后，肝脏中持续的游离型转基因表达

A Human Papillomavirus (HPV) - basedpseudoviral gene delivery system for the non-viral,Episomally Replicating Vector pEPI-1

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