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首页> 外文期刊>Gene Therapy and Molecular Biology >Segregation of partly melted molecules and its application to the isolation of methylated CpG islands in human cancer cells
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Segregation of partly melted molecules and its application to the isolation of methylated CpG islands in human cancer cells

机译：部分熔融分子的分离及其在人类癌细胞甲基化CpG岛分离中的应用

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Segregation of partly melted molecules is a convenient and efficient method for isolation of DNA fragments associated with CpG islands. DNA fragments digested with restriction endonucleases are subjected to denaturing gradient gel electrophoresis. DNA fragments derived from CpG islands are preferentially retained in the gel after prolonged field exposure because of their lower rate of strand dissociation. An independent technique, methylated-DNA-binding column chromatography, permits separation of DNA fragments on the basis of the number of methyl-CpG sequences in the fragment, and it enables separation of methylated CpG islands from those that are not methylated. Segregation of partly melted molecules and methylated-DNA-binding column chromatography were successfully combined to isolate CpG islands methylated in human adenocarcinomas of the lung. The methylated CpG island library will be valuable in order to elucidate epigenetic process in carcinogenesis.
机译：部分熔融分子的分离是分离与CpG岛相关的DNA片段的便捷有效方法。用限制性核酸内切酶消化的DNA片段经过变性梯度凝胶电泳。源自CpG岛的DNA片段在长时间的现场暴露后会优先保留在凝胶中，因为它们的链解离速率较低。一项独立的技术，甲基化DNA结合柱色谱法，允许根据片段中甲基CpG序列的数量分离DNA片段，并且可以将甲基化CpG岛与未甲基化的岛分离。成功地将部分熔融的分子分离和甲基化-DNA结合柱层析结合起来，以分离人肺腺癌中甲基化的CpG岛。甲基化的CpG岛文库对于阐明癌发生过程中的表观遗传过程将是有价值的。

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