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首页> 外文期刊>Genes >The Quorum Sensing System of Yersinia enterocolitica 8081 Regulates Swimming Motility, Host Cell Attachment, and Virulence Plasmid Maintenance
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The Quorum Sensing System of Yersinia enterocolitica 8081 Regulates Swimming Motility, Host Cell Attachment, and Virulence Plasmid Maintenance

机译：耶尔森氏菌肠球菌8081的群体感应系统调节游泳运动，宿主细胞附着和毒力质粒维持

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Although Yersinia enterocolitica genomes are highly heterogeneous, they contain a conserved N -acylhomoserine lactone-dependent (AHL) quorum sensing (QS) system consisting of the luxR and luxI orthologs yenR and yenI respectively. Certain hypervirulent strains also contain a putative orphan luxR gene, ycoR , that is not linked to an AHL synthase. To explore the contribution of yenR/yenI/ycoR to QS-dependent phenotypes in Yersinia enterocolitica strain 8081, single and multiple mutants were constructed. AHL profiling identified N -(3-oxohexanoyl) homoserine lactone, N -hexanoylhomoserine lactone, and N -(3-oxoseptanoyl) homoserine lactone as the most abundant. The AHL profiles of the yenR , ycoR and yenR/ycoR mutants were similar to the parent suggesting that the two LuxR homologues do not regulate AHL production while the yenI mutants were AHL-negative. A role for QS in swimming motility and cell attachment was demonstrated. Down-regulation of the virulence plasmid partition gene, spyA , in yenI and yenI / yenR / ycoR mutants is consistent with the greater loss of the Y. enterocolitica pYVe virulence plasmid in the yenI mutant during serial passage at 37 °C but not at 22 °C. A role for QS-regulated spyA in virulence plasmid maintenance is suggested.
机译：尽管小肠结肠炎耶尔森氏菌基因组高度异质，但它们包含保守的N-酰基高丝氨酸内酯依赖性（AHL）群体感应（QS）系统，分别由luxR和luxI直系同源物JPYR和yenI组成。某些高毒力菌株还含有未与AHL合酶连接的推定的孤儿luxR基因ycoR。为了探索日元R / yenI / ycoR对小肠结肠炎耶尔森氏菌菌株8081中QS依赖表型的贡献，构建了单个和多个突变体。 AHL分析表明，N-（3-氧代己酰基）高丝氨酸内酯，N-己酸高丝氨酸内酯和N-（3-氧代庚酰基）高丝氨酸内酯含量最高。 yenR，ycoR和yenR / ycoR突变体的AHL谱与亲本相似，表明这两个LuxR同源物不能调节AHL的产生，而yenI突变体却是AHL阴性的。证明了QS在游泳运动和细胞附着中的作用。在Y型和Y型I / yenR / ycoR突变体中毒力质粒分配基因spyA的下调与在37°C而不是在22°C的连续传代期间日元I突变体中肠球菌pYVe毒力质粒的更大损失相一致℃。建议在毒力质粒维持中QS调节的spyA的作用。

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