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首页> 外文期刊>Genes and Nutrition >Bioinformatics for the NuGO proof of principle study: analysis of gene expression in muscle of ApoE3*Leiden mice on a high-fat diet using PathVisio
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Bioinformatics for the NuGO proof of principle study: analysis of gene expression in muscle of ApoE3*Leiden mice on a high-fat diet using PathVisio

机译：NuGO原理研究证明的生物信息学：使用PathVisio分析高脂饮食下ApoE3 * Leiden小鼠肌肉中的基因表达

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摘要

Insulin resistance is a characteristic of type-2 diabetes and its development is associated with an increased fat consumption. Muscle is one of the tissues that becomes insulin resistant after high fat (HF) feeding. The aim of the present study is to identify processes involved in the development of HF-induced insulin resistance in muscle of ApOE3*Leiden mice by using microarrays. These mice are known to become insulin resistant on a HF diet. Differential gene expression was measured in muscle using the Affymetrix mouse plus 2.0 array. To get more insight in the processes, affected pathway analysis was performed with a new tool, PathVisio. PathVisio is a pathway editor customized with plug-ins (1) to visualize microarray data on pathways and (2) to perform statistical analysis to select pathways of interest. The present study demonstrated that with pathway analysis, using PathVisio, a large variety of processes can be investigated. The significantly regulated genes in muscle of ApOE3*Leiden mice after 12?weeks of HF feeding were involved in several biological pathways including fatty acid beta oxidation, fatty acid biosynthesis, insulin signaling, oxidative stress and inflammation.
机译：胰岛素抵抗是2型糖尿病的特征，其发展与脂肪消耗增加有关。肌肉是高脂（HF）喂养后对胰岛素产生抵抗力的组织之一。本研究的目的是通过使用微阵列来鉴定与ApOE3 * Leiden小鼠肌肉中HF诱导的胰岛素抵抗发展有关的过程。已知这些小鼠在HF饮食中会产生胰岛素抵抗。使用Affymetrix小鼠plus 2.0阵列测量肌肉中的差异基因表达。为了获得对流程的更多了解，使用新工具PathVisio进行了受影响的路径分析。 PathVisio是使用插件定制的途径编辑器，其插件（1）可以使途径上的微阵列数据可视化，以及（2）可以进行统计分析以选择感兴趣的途径。本研究表明，使用PathVisio进行路径分析后，可以研究各种各样的过程。 HF喂养12周后，ApOE3 * Leiden小鼠肌肉中显着调节的基因参与了几种生物途径，包括脂肪酸β氧化，脂肪酸生物合成，胰岛素信号传导，氧化应激和炎症。

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