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首页> 外文期刊>Genetics and molecular biology: publication of the Sociedade Brasileira de Genetica >Molecular cloning, characterization and expression analysis of a novel PDRG1 gene from black tiger shrimp (Penaeus monodon)
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Molecular cloning, characterization and expression analysis of a novel PDRG1 gene from black tiger shrimp (Penaeus monodon)

机译：黑虎对虾PDRG1新基因的分子克隆，鉴定和表达分析

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摘要

P53 And DNA Damage-Regulated Gene 1 (PDRG1) is a novel gene which plays an important role in chaperone-mediated protein folding. In the present study, the full-length complementary DNA (cDNA) sequence of the PDRG1 gene from Penaeus monodon ( PmPDRG1 ) was cloned by the rapid amplification of cDNA ends (RACE) method. The cDNA of PmPDRG1 spans 1,613 bp, interrupted by only one short intron, and encodes a protein of 136 amino acids with calculated molecular weight of 15.49 kDa. The temporal expression profile of PmPDRG1 in different tissues and in different developmental stages of the ovary was investigated by real-time quantitative PCR (RT-qPCR). An RNA interference (RNAi) experiment was performed to study the relationship between P. monodon p53 ( Pmp53 ) and PmPDRG1 , and the results showed that the relative expression level of PmPDRG1 mRNA was notably up-regulated from 12 h to 96 h after Pmp53 was silenced both in ovary and hepatopancreas. To further explore the role of PmPDRG1 in ovarian development, dopamine (DA) and 5-hydroxytryptamine (5-HT)-injected shrimps were analyzed by RT-qPCR, indicating that PmPDRG1 may be involved in the regulation of ovarian development of P. monodon .
机译：P53和DNA损伤调控基因1（PDRG1）是一种新基因，在伴侣蛋白介导的蛋白质折叠中起着重要作用。在本研究中，通过快速扩增cDNA末端（RACE）方法克隆了斑节对虾PDRG1基因的全长互补DNA（cDNA）序列（PmPDRG1）。 PmPDRG1的cDNA跨度为1,613 bp，仅被一个短内含子打断，编码136个氨基酸的蛋白质，计算分子量为15.49 kDa。通过实时定量PCR（RT-qPCR）研究了PmPDRG1在卵巢的不同组织和不同发育阶段的时间表达谱。进行了RNA干扰（RNAi）实验，研究了斑节对虾p53（Pmp53）与PmPDRG1的关系，结果表明，从Pmp53表达12h至96h，PmPDRG1 mRNA的相对表达水平显着上调。卵巢和肝胰腺均无声。为了进一步探讨PmPDRG1在卵巢发育中的作用，通过RT-qPCR分析了注射多巴胺（DA）和5-羟色胺（5-HT）的虾，表明PmPDRG1可能参与了斑节对虾的卵巢发育调控。 。

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