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Towards establishing capacity for biological dosimetry at ghana atomic energy commission

机译:在加纳原子能委员会建立生物剂量学的能力

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The aim of this study was not only to obtain basic technical prerequisites for the establishment of capacity of biological dosimetry at the Ghana Atomic Energy Commission (GAEC) but also to stimulate interest in biological dosimetry research in Ghana and Sub-Saharan Africa. Peripheral blood from four healthy donors was exposed to different doses (0–6 Gy) of gamma rays from a radiotherapy machine and lymphocytes were subsequently stimulated, cultured, and processed according to standard protocols for 48–50 h. Processed cells were analyzed for the frequencies of dicentric and centric ring chromosomes. Radiation dose delivered to the experimental model was verified using GafChromic? EBT films in parallel experiments. Basic technical prerequisites for the establishment of capacity of biological dosimetry in the GAEC have been realized and expertise in the dicentric chromosome assay consolidated. We successfully obtained preliminary cytogenetic data for a dose-response relationship of the irradiated blood lymphocytes. The data strongly indicate the existence of significant linear (α) and quadratic (β) components and are consistent with those published for the production of chromosome aberrations in comparable absorbed dose ranges.
机译:这项研究的目的不仅是获得加纳原子能委员会(GAEC)建立生物剂量测定能力的基本技术前提,而且还激发了加纳和撒哈拉以南非洲对生物剂量测定研究的兴趣。来自四名健康供体的外周血暴露于放射治疗机的不同剂量(0-6 Gy)的γ射线,随后按照标准规程刺激,培养和处理淋巴细胞48-50小时。分析处理过的细胞的双中心和中心环染色体的频率。使用GafChromic?验证了传递到实验模型的辐射剂量。并行实验中的EBT胶片。 GAEC建立生物剂量测定能力的基本技术先决条件已经实现,双中心染色体测定的专业知识得到了巩固。我们成功地获得了辐射血淋巴细胞剂量-反应关系的初步细胞遗传学数据。数据强烈表明存在重要的线性(α)和二次(β)成分,并且与在相当的吸收剂量范围内产生染色体畸变的出版物一致。

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