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首页> 外文期刊>Genome Integrity >Genome-wide distribution of histone H4 Lysine 16 acetylation sites and their relationship to gene expression
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Genome-wide distribution of histone H4 Lysine 16 acetylation sites and their relationship to gene expression

机译：组蛋白H4赖氨酸16个乙酰化位点的全基因组分布及其与基因表达的关系

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Background Histone post-translational modifications are critical determinants of chromatin structure and function, impacting multiple biological processes including DNA transcription, replication, and repair. The post-translational acetylation of histone H4 at lysine 16 (H4K16ac) was initially identified in association with dosage compensation of the Drosophila male X chromosome. However, in mammalian cells, H4K16ac is not associated with dosage compensation and the genomic distribution of H4K16ac is not precisely known. Therefore, we have mapped the genome-wide H4K16ac distribution in human cells. Results We performed H4K16ac chromatin immunoprecipitation from human embryonic kidney 293 (HEK293) cells followed by hybridization to whole-genome tiling arrays and identified 25,893 DNA regions (false discovery rate ±1.5-fold) were identified as potential H4K16ac-regulated genes. Seventeen transcription factor-binding sites were significantly associated with H4K16ac occupancy (p? Conclusions The results suggest that H4K16 acetylation has a limited effect on transcription regulation in HEK293 cells, whereas H4K16ac has been demonstrated to have critical roles in regulating transcription in mouse embryonic stem cells. Thus, H4K16ac-dependent transcription regulation is likely a cell type specific process.
机译：背景组蛋白翻译后修饰是染色质结构和功能的关键决定因素，影响多种生物学过程，包括DNA转录，复制和修复。最初鉴定出组蛋白H4在赖氨酸16（H4K16ac）的翻译后乙酰化与果蝇雄性X染色体的剂量补偿有关。但是，在哺乳动物细胞中，H4K16ac与剂量补偿无关，并且H4K16ac的基因组分布尚不清楚。因此，我们绘制了人类细胞中全基因组H4K16ac分布图。结果我们从人胚肾293（HEK293）细胞中进行了H4K16ac染色质免疫沉淀，然后与全基因组切片阵列杂交，鉴定出25,893个DNA区域（错误发现率±1.5倍）被鉴定为潜在的H4K16ac调控基因。 17个转录因子结合位点与H4K16ac的占有率显着相关（p？结论）结果表明，H4K16乙酰化对HEK293细胞的转录调控作用有限，而H4K16ac已被证明在调节小鼠胚胎干细胞的转录中起关键作用。因此，依赖H4K16ac的转录调控可能是细胞类型特异性过程。

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