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首页> 外文期刊>Genetics and Molecular Research >Sequence characterization, polymorphism, and tissue expression profile of an effector immediate-early gene: activity-regulated cytoskeletal associated protein gene (Arc/Arg3.1) in swamp and river buffalo
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Sequence characterization, polymorphism, and tissue expression profile of an effector immediate-early gene: activity-regulated cytoskeletal associated protein gene (Arc/Arg3.1) in swamp and river buffalo

机译：效应器立即早期基因的序列表征，多态性和组织表达谱：沼泽和河水牛中活性调节的细胞骨架相关蛋白基因（Arc / Arg3.1）

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The activity-regulated cytoskeletal associated protein (Arc/Arg3.1) has been implicated in experience-dependent synaptic plasticity and memory formation. However, information regarding its coding gene in buffalo remains scarce. In this study, the full-length of Arc/Arg3.1 was isolated and characterized (accession No. JX491649) and genetic variations of six river buffalo and eight swamp buffalo were investigated. A tissue expression profile was obtained using semi-quantitative reverse transcription-polymerase chain reaction. The coding region sequence of Arc/Arg3.1 contained 1191 nucleotides encoding a putative protein of 396 amino acids with a theoretical isoelectric point (pI) and molecular weight (Mw) of 5.4 and 45.2 kDa, respectively. Four polymorphisms (c.63TC, c.228TC, c.558GA, and c.625GC) were found in buffalo; however, only substitution c.625GC was non-synonymous, leading to an amino acid change from Val to Leu at the 209th position of the Arc/Arg3.1 protein sequence. Bioinformatics analysis revealed that this substitution had no significant effect on Arc/Arg3.1 function (subPSEC = -1.4039, Pdeleterious = 0.1685), which indicated that Arc/Arg3.1 was highly conserved and functionally important in buffalo. Phylogenetic analysis revealed that the gene is closely related to that of Bos taurus and Bos grunniens. The gene was moderately expressed in the hypophysis and the placenta; it was weakly expressed in the kidney, milk, mammary gland, cerebrum, lung, heart, rumen, fat, and uterus; and it was almost silent in the muscle, liver, and skin. These findings will provide further insights into the structure and function of the immediate-early gene in buffalo.
机译：活动调节的细胞骨架相关蛋白（Arc / Arg3.1）已牵涉经验依赖的突触可塑性和记忆形成。但是，关于其在水牛中的编码基因的信息仍然很少。在本研究中，分离并鉴定了全长Arc / Arg3.1（登录号JX491649），并研究了6个河水牛和8个沼泽水牛的遗传变异。使用半定量逆转录-聚合酶链反应获得组织表达谱。 Arc / Arg3.1的编码区序列包含1191个核苷酸，编码396个氨基酸的推定蛋白质，理论等电点（pI）和分子量（Mw）分别为5.4和45.2 kDa。在水牛中发现了四个多态性（c.63T> C，c.228T> C，c.558G> A和c.625G> C）。然而，只有取代c.625G> C是非同义的，导致在Arc / Arg3.1蛋白序列的第209位氨基酸从Val变为Leu。生物信息学分析表明，这种取代对Arc / Arg3.1功能没有显着影响（subPSEC = -1.4039，Pdeleterious = 0.1685），这表明Arc / Arg3.1在水牛中是高度保守的且在功能上很重要。系统发育分析表明，该基因与金牛座和格氏牛紧密相关。该基因在垂体和胎盘中适度表达。它在肾脏，牛奶，乳腺，大脑，肺，心脏，瘤胃，脂肪和子宫中表达较弱;肌肉，肝脏和皮肤几乎保持沉默。这些发现将为水牛中早期基因的结构和功能提供进一步的见识。

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