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首页> 外文期刊>Genetics and Molecular Research >Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica
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Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica

机译：结缕草的叶绿素降解调控基因（ZjSGR）的分子克隆与鉴定

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The stay-green gene (SGR) is a key regulatory factor for chlorophyll degradation and senescence. However, to date, little is known about SGR in Zoysia japonica. In this study, ZjSGR was cloned, using rapid amplification of cDNA ends-polymerase chain reaction (PCR). The target sequence is 831 bp in length, corresponding to 276 amino acids. Protein BLAST results showed that ZjSGR belongs to the stay-green superfamily. A phylogenetic analysis implied that ZjSGR is most closely related to ZmSGR1. The subcellular localization of ZjSGR was investigated, using an Agrobacterium-mediated transient expression assay in Nicotiana benthamiana. Our results demonstrated that ZjSGR protein is localized in the chloroplasts. Quantitative real time PCR was carried out to investigate the expression characteristics of ZjSGR. The expression level of ZjSGR was found to be highest in leaves, and could be strongly induced by natural senescence, darkness, abscisic acid (ABA), and methyl jasmonate treatment. Moreover, an in vivo function analysis indicated that transient overexpression of ZjSGR could accelerate chlorophyll degradation, up-regulate the expression of SAG113, and activate ABA biosynthesis. Taken together, these results provide evidence that ZjSGR could play an important regulatory role in leaf chlorophyll degradation and senescence in plants at the molecular level.
机译：保持绿色基因（SGR）是叶绿素降解和衰老的关键调控因子。但是，迄今为止，关于结缕草的SGR知之甚少。在这项研究中，通过快速扩增cDNA末端聚合酶链反应（PCR）克隆了ZjSGR。靶序列的长度为831bp，对应于276个氨基酸。蛋白质BLAST结果表明ZjSGR属于保持绿色的超家族。系统发育分析表明ZjSGR与ZmSGR1最密切相关。使用农杆菌介导的本生烟草中的瞬时表达测定法研究了ZjSGR的亚细胞定位。我们的结果证明ZjSGR蛋白位于叶绿体中。进行实时定量PCR研究ZjSGR的表达特征。发现ZjSGR的表达水平在叶片中最高，并且可以由自然衰老，黑暗，脱落酸（ABA）和茉莉酸甲酯处理强烈诱导。此外，体内功能分析表明，ZjSGR的瞬时过表达可以加速叶绿素降解，上调SAG113的表达，并激活ABA的生物合成。综上所述，这些结果提供了证据，表明ZjSGR可以在分子水平上在植物叶片叶绿素的降解和衰老中发挥重要的调节作用。

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