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首页> 外文期刊>Genomics Insights >Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid:
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Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid:

机译：几内亚油菜和油菜及其种间杂种的基因组大小的重新评估：

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Aiming at generating a comprehensive genomic database on Elaeis spp., our group is leading several R&D initiatives with Elaeis guineensis (African oil palm) and Elaeis oleifera (American oil palm), including the whole-genome sequencing of the last. Genome size estimates currently available for this genus are controversial, as they indicate that American oil palm genome is about half the size of the African oil palm genome and that the genome of the interspecific hybrid is bigger than both the parental species genomes. We estimated the genome size of three E. guineensis genotypes, five E. oleifera genotypes, and two interspecific hybrids genotypes. On average, the genome size of E. guineensis is 4.32 ± 0.173 pg, while that of E. oleifera is 4.43 ± 0.018 pg. This indicates that both genomes are similar in size, even though E. oleifera is in fact bigger. As expected, the hybrid genome size is around the average of the two genomes, 4.40 ± 0.016 pg. Additionally, we demonstrate that both species present around 38% of GC content. As our results contradict the currently available data on Elaeis spp. genome sizes, we propose that the actual genome size of the Elaeis species is around 4 pg and that American oil palm possesses a larger genome than African oil palm.
机译：为了在Elaeis spp。上生成一个全面的基因组数据库，我们小组领导着Elaeis guineensis（非洲油棕）和Elaeis oleifera（美国油棕）的多项研发计划，包括最后一个的全基因组测序。目前可用于该属的基因组大小估计是有争议的，因为它们表明美国油棕基因组大约是非洲油棕基因组大小的一半，种间杂种的基因组大于两个亲本物种基因组。我们估计了三种大肠杆菌基因型，五种油菜基因型和两种种间杂种基因型的基因组大小。平均而言，吉尼斯E. guineensis的基因组大小为4.32±0.173 pg，而大肠埃希菌的基因组大小为4.43±0.018 pg。这表明两个基因组在大小上都相似，即使油橄榄实际上更大。正如预期的那样，杂种基因组的大小约为两个基因组的平均值，即4.40±0.016 pg。此外，我们证明了这两个物种均约占GC含量的38％。由于我们的结果与Elaeis spp上的当前可用数据相矛盾。基因组大小，我们提出，Elaeis物种的实际基因组大小约为4 pg，美国油棕比非洲油棕拥有更大的基因组。

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