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首页> 外文期刊>Genomics >Comparative transcriptomics of Pleurotus eryngii reveals blue-light regulation of carbohydrate-active enzymes (CAZymes) expression at primordium differentiated into fruiting body stage
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Comparative transcriptomics of Pleurotus eryngii reveals blue-light regulation of carbohydrate-active enzymes (CAZymes) expression at primordium differentiated into fruiting body stage

机译：杏鲍菇侧耳的比较转录组学揭示了在分化为子实体期的原基上碳水化合物活性酶（CAZymes）表达的蓝光调节

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摘要

Blue light is an important environmental factor which could induce mushroom primordium differentiation and fruiting body development. However, the mechanisms of Pleurotus eryngii primordium differentiation and development induced by blue light are still unclear. The CAZymes (carbohydrate-active enzymes) play important roles in degradation of renewable lignocelluloses to provide carbohydrates for fungal growth, development and reproduction. In the present research, the expression profiles of genes were measured by comparison between the Pleurotus eryngii at primordium differentiated into fruiting body stage after blue light stimulation and dark using high-throughput sequencing approach. After assembly and compared to the Pleurotus eryngii reference genome, 11,343 unigenes were identified. 539 differentially expressed genes including white collar 2 type of transcription factor gene, A mating type protein gene, MAP kinase gene, oxidative phosphorylation associated genes, CAZymes genes and other metabolism related genes were identified during primordium differentiated into fruiting body stage after blue light stimulation. KEGG results showed that carbon metabolism, glycolysis/gluconeogenesis and biosynthesis of amino acids pathways were affected during blue light inducing primordia formation. Most importantly, 319 differentially expressed CAZymes participated in carbon metabolism were identified. The expression patterns of six representative CAZymes and laccase genes were further confirmed by qRT-PCR. Enzyme activity results indicated that the activities of CAZymes and laccase were affected in primordium differentiated into fruiting body under blue light stimulation. In conclusion, the comprehensive transcriptome and CAZymes of Pleurotus eryngii at primordium differentiated into fruiting body stage after blue light stimulation were obtained. The biological insights gained from this integrative system represent a valuable resource for future genomic studies on this commercially important mushroom.
机译：蓝光是可能导致蘑菇原基分化和子实体发育的重要环境因素。然而，蓝光引起的杏鲍菇原基分化和发育的机制仍不清楚。 CAZymes（碳水化合物活性酶）在可再生木质纤维素降解中起重要作用，从而为真菌的生长，发育和繁殖提供碳水化合物。在本研究中，通过使用高通量测序方法，通过比较在蓝光刺激后和黑暗后分化成子实体期的原基平菇中的杏鲍菇侧耳的基因表达谱。组装后，与杏鲍菇参考基因组进行比较，鉴定出11,343个单基因。蓝光刺激后原基分化为子实体期，鉴定出539个差异表达基因，包括白领2型转录因子基因，A交配型蛋白基因，MAP激酶基因，氧化磷酸化相关基因，CAZymes基因及其他代谢相关基因。 KEGG结果表明，在蓝光诱导原基形成过程中，碳代谢，糖酵解/糖异生和氨基酸途径的生物合成受到影响。最重要的是，鉴定了319个差异表达的CAZymes参与了碳代谢。通过qRT-PCR进一步证实了六个代表性CAZymes和漆酶基因的表达模式。酶活性结果表明，在蓝光刺激下，分化为子实体的原基中CAZymes和漆酶的活性受到影响。结论：蓝光刺激后，杏鲍菇侧耳原基的完整转录组和CAZymes已分化为子实体期。从这种整合系统中获得的生物学见解为将来对该具有重要商业意义的蘑菇进行基因组研究提供了宝贵的资源。

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