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首页> 外文期刊>G3: Genes, Genomes, Genetics >A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas × Crassostrea angulata) Using Genotyping-by-Sequencing
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A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas × Crassostrea angulata) Using Genotyping-by-Sequencing

机译：高密度SNP遗传连锁图谱和牡蛎（Crassostrea gigas×Crassostrea angulata）杂交牡蛎生长相关性状的QTL分析

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摘要

Oysters are among the most important species in global aquaculture. Crassostrea gigas , and its subspecies C. angulata , are the major cultured species. To determine the genetic basis of growth-related traits in oysters, we constructed a second-generation linkage map from 3367 single-nucleotide polymorphisms (SNPs) based on genotyping-by-sequencing, genotyped from a C.?gigas ?×? C.?angulata hybrid family. These 3367 SNPs were distributed on 1695 markers, which were assigned to 10 linkage groups. The genetic linkage map had a total length of 1084.3?cM, with an average of 0.8?cM between markers; it thus represents the densest genetic map constructed for oysters to date. Twenty-seven quantitative trait loci (QTL) for five growth-related traits were detected. These QTL could explain 4.2–7.7% (mean?=?5.4%) of the phenotypic variation. In total, 50.8% of phenotypic variance for shell width, 7.7% for mass weight, and 34.1% for soft tissue weight were explained. The detected QTL were distributed among eight linkage groups, and more than half (16) were concentrated within narrow regions in their respective linkage groups. Thirty-eight annotated genes were identified within the QTL regions, two of which are key genes for carbohydrate metabolism. Other genes were found to participate in assembly and regulation of the actin cytoskeleton, signal transduction, and regulation of cell differentiation and development. The newly developed high-density genetic map, and the QTL and candidate genes identified provide a valuable genetic resource and a basis for marker-assisted selection for C.?gigas and C.?angulata .
机译：牡蛎是全球水产养殖中最重要的物种之一。 Crassostrea gigas及其亚种C. angulata是主要的养殖物种。为了确定牡蛎中与生长相关的性状的遗传基础，我们基于按基因分型，从C.?gigas?×？基因分型的3367个单核苷酸多态性（SNP）构建了第二代连锁图谱。 C.?angulata杂交家庭。这3367个SNP分布在1695个标记上，这些标记被分配给10个连锁组。遗传连锁图的总长度为1084.3？cM，标记之间的平均长度为0.8？cM。因此，它代表了迄今为止为牡蛎构建的最密集的遗传图谱。检测到五个与生长相关的性状的27个数量性状位点（QTL）。这些QTL可以解释表型变异的4.2–7.7％（平均？=？5.4％）。总共解释了表壳宽度的50.8％，质量重量的7.7％和软组织重量的34.1％。检测到的QTL分布在八个连锁组中，一半以上（16）集中在各自连锁组的狭窄区域内。在QTL区域内鉴定了38个带注释的基因，其中两个是碳水化合物代谢的关键基因。发现其他基因参与肌动蛋白细胞骨架的组装和调节，信号转导以及细胞分化和发育的调节。新近开发的高密度遗传图谱，以及鉴定出的QTL和候选基因为珍贵的C.?gas和C.?angulata提供了宝贵的遗传资源和标记辅助选择的基础。

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