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首页> 外文期刊>Genome Integrity >The frequency of lymphocytes containing dumbbell-shaped nuclei depends on ionizing radiation dose and correlates with appearance of chromosomal aberrations
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The frequency of lymphocytes containing dumbbell-shaped nuclei depends on ionizing radiation dose and correlates with appearance of chromosomal aberrations

机译：包含哑铃形核的淋巴细胞的频率取决于电离辐射剂量，并与染色体畸变的出现相关

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摘要

Nuclear anomalies of different types appear in cells in response to the action of ionizing radiation after the passage of the first mitotic division. In this article, we present the results of the study of the frequency of occurrence of three types of nuclear anomalies (“tailed” nuclei, nucleoplasmic bridges, and dumbbell-shaped nuclei) in vitro in human lymphocytes cultured with cytochalasin B when exposed to X-rays at doses of 0.0, 0.1, 0.2, 0.4, 0.5, 0.7, 1.0, 1.5, and 2.0 Gy. To stop the cell cycle of cultured lymphocytes after the first mitotic division, a cytokinesis block was performed using cytochalasin B. Dose-dependent curves of the occurrence of lymphocytes containing “tailed” nuclei, nucleoplasmic bridges, or dumbbell-shaped nuclei after irradiation have been constructed. At the same time, frequencies of occurrence of chromosomal aberrations (dicentric and ring chromosomes) in the culture of lymphocytes exposed to the same radiation doses were studied. Comparison of the frequencies of occurrence of dicentric and ring chromosomes with frequencies of occurrence of nuclear anomalies allows us to conclude that these nuclear anomalies are formed as a result of chromosomal aberrations arising in lymphocytes under the action of ionizing radiation. More than that, most of the chromosomal aberrations are converted into dumbbell-shaped nuclei in vitro in the culture of lymphocytes in the cytochalasin block.
机译：在第一次有丝分裂分裂之后，响应于电离辐射的作用，细胞中会出现不同类型的核异常。在本文中，我们介绍了三种细胞核素（“尾”核，核质桥和哑铃形核）在暴露于X的细胞松弛素中体外发生的频率的体外研究结果以0.0、0.1、0.2、0.4、0.5、0.7、1.0、1.5和2.0 Gy的剂量照射。为了停止第一次有丝分裂分裂后培养的淋巴细胞的细胞周期，使用细胞松弛素B进行胞质分裂阻滞。照射后，含有“尾状”核，核质桥或哑铃状核的淋巴细胞的剂量依赖性曲线已被确定。建。同时，研究了暴露于相同辐射剂量的淋巴细胞培养物中染色体畸变（双着丝粒和环状染色体）的发生频率。比较双着丝粒和环状染色体的发生频率与核异常发生的频率可以得出结论，这些核异常是由于电离辐射作用下淋巴细胞中产生的染色体畸变而形成的。不仅如此，在细胞松弛素区的淋巴细胞培养物中，大多数染色体畸变在体外都转化为哑铃状核。

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