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首页> 外文期刊>IUCrJ >Single-particle imaging without symmetry constraints at an X-ray free-electron laser
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Single-particle imaging without symmetry constraints at an X-ray free-electron laser

机译：X射线自由电子激光器无对称约束的单粒子成像

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摘要

The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.
机译：作为SPI计划的一部分，本文介绍了在LCLS的AMO光束线上进行的单粒子成像（SPI）实验的分析。开发了从测量的单颗粒数据对PR772噬菌体进行三维病毒重建的工作流程。它包括几个明确定义的步骤，包括单次命中衍射数据分类，分类数据的精细过滤，通过方向确定从实验衍射图重建三维散射强度以及最终不进行病毒电子密度的三维重建。对称约束。此处进行的分析揭示并量化了该实验中PR772病毒的纳米级特征，获得的分辨率优于10 nm，清楚地表明该结构在一个方向上被压缩，因此偏离了理想的二十面体对称性。

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