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Synchrotron radiation macromolecular crystallography: science and spin-offs

机译：同步辐射大分子晶体学：科学和附带利益

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摘要

A current overview of synchrotron radiation (SR) in macromolecular crystallography (MX) instrumentation, methods and applications is presented. Automation has been and remains a central development in the last decade, as have the rise of remote access and of industrial service provision. Results include a high number of Protein Data Bank depositions, with an increasing emphasis on the successful use of microcrystals. One future emphasis involves pushing the frontiers of using higher and lower photon energies. With the advent of X-ray free-electron lasers, closely linked to SR developments, the use of ever smaller samples such as nanocrystals, nanoclusters and single molecules is anticipated, as well as the opening up of femtosecond time-resolved diffraction structural studies. At SR sources, a very high-throughput assessment for the best crystal samples and the ability to tackle just a few micron and sub-micron crystals will become widespread. With higher speeds and larger detectors, diffraction data volumes are becoming long-term storage and archiving issues; the implications for today and the future are discussed. Together with the rise of the storage ring to its current pre-eminence in MX data provision, the growing tendency of central facility sites to offer other centralized facilities complementary to crystallography, such as cryo-electron microscopy and NMR, is a welcome development.
机译：本文介绍了大分子晶体学（MX）仪器中同步加速器辐射（SR）的最新概况，方法和应用。在过去的十年中，自动化一直是并且仍然是中心发展，远程访问和工业服务提供的兴起也是如此。结果包括大量的蛋白质数据库沉积，并越来越重视成功使用微晶。未来的重点之一是推动使用更高和更低的光子能量的前沿。随着与SR的发展紧密相关的X射线自由电子激光器的出现，人们期望使用更小的样品，例如纳米晶体，纳米团簇和单分子，以及飞秒时间分辨衍射结构研究的开始。在SR来源，对最佳晶体样品的高通量评估以及仅处理几微米和亚微米晶体的能力将变得广泛。随着更高的速度和更大的检测器，衍射数据量已成为长期的存储和归档问题。讨论了对当今和未来的影响。随着存储环在MX数据提供中的地位日趋突出，中央设施站点提供与晶体学互补的其他中央设施（如冷冻电子显微镜和NMR）的趋势日益增长，这是一个可喜的发展。

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