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首页> 外文期刊>IUCrJ >Crystal structure of peroxiredoxin 3 from Vibrio vulnificus and its implications for scavenging peroxides and nitric oxide
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Crystal structure of peroxiredoxin 3 from Vibrio vulnificus and its implications for scavenging peroxides and nitric oxide

机译：创伤弧菌Peroxiredoxin 3的晶体结构及其对清除过氧化物和一氧化氮的影响

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Peroxiredoxins (Prxs) are ubiquitous cysteine-based peroxidase enzymes. Recently, a new type of Prx, VvPrx3, was identified in the pathogenic bacterium Vibrio vulnificus as being important for survival in macrophages. It employs only one catalytic cysteine residue to decompose peroxides. Here, crystal structures of VvPrx3 representing its reduced and oxidized states have been determined, together with an H2O2-bound structure, at high resolution. The crystal structure representing the reduced Prx3 showed a typical dimeric interface, called the A-type interface. However, VvPrx3 forms an oligomeric interface mediated by a disulfide bond between two catalytic cysteine residues from two adjacent dimers, which differs from the doughnut-like oligomers that appear in most Prxs. Subsequent biochemical studies showed that this disulfide bond was induced by treatment with nitric oxide (NO) as well as with peroxides. Consistently, NO treatment induced expression of the prx3 gene in V. vulnificus, and VvPrx3 was crucial for the survival of bacteria in the presence of NO. Taken together, the function and mechanism of VvPrx3 in scavenging peroxides and NO stress via oligomerization are proposed. These findings contribute to the understanding of the diverse functions of Prxs during pathogenic processes at the molecular level.
机译：过氧化物酶（Prxs）是普遍存在的基于半胱氨​​酸的过氧化物酶。最近，在致病性弧菌弧菌中发现了一种新型的Prx VvPrx3，它对巨噬细胞的存活很重要。它仅使用一个催化的半胱氨酸残基分解过氧化物。在这里，已经确定了代表VvPrx3还原和氧化态的晶体结构，以及与H2O2结合的结构，并且分辨率很高。代表还原的Prx3的晶体结构显示出典型的二聚体界面，称为A型界面。但是，VvPrx3形成一个寡聚界面，该界面由来自两个相邻二聚体的两个催化半胱氨酸残基之间的二硫键介导，这不同于大多数Prx中出现的甜甜圈状低聚物。随后的生化研究表明，该二硫键是通过一氧化氮（NO）和过氧化物处理而诱导的。一致地，NO处理诱导了创伤弧菌中prx3基因的表达，而VvPrx3对于在NO存在下细菌的存活至关重要。总之，提出了VvPrx3通过低聚清除过氧化物和NO胁迫的功能和机理。这些发现有助于在分子水平的致病过程中理解Prxs的各种功能。

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