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首页> 外文期刊>Global Journal of Research on Medicinal Plants and Indigenous Medicine >MOLECULAR MARKER STUDIES OF SOME MEDICINAL PLANTS FOR ASSESSMENT OF GENETIC DIVERSITY AND RELATIONSHIPS
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MOLECULAR MARKER STUDIES OF SOME MEDICINAL PLANTS FOR ASSESSMENT OF GENETIC DIVERSITY AND RELATIONSHIPS

机译：用于评估遗传多样性和亲缘关系的某些药用植物的分子标记研究

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Genetic relationships between eighteen medicinal were assayed with Random Amplified Polymorphic DNA (RAPD) markers, which distinguish individuals, as well as reflecting the inherent variation and interrelationships among the medicinal plants. 13 decamer RAPD primers were used in the present study. Over 77.23 reproducible bands were generated by RAPD primers, out of which, 77.23 polymorphic bands were identified, conferring 97.6% polymorphism. All the primers produced typical banding in each of the medicinal plants, suggesting the applicability of this test in medicinal plant identification. Most of the individuals of the test exhibited to have unique molecular genotype. Population genetics structure analysis of these species further revealed high genetic differentiation coefficients (Gst), the heterozygosity among populations (Ht) showed with the low gene flow (Nm) when 1 st cluster was paired with other medicinal plants On the basis of these parameters and the results of cluster analysis. A dendrogram was constructed using Euclidean distance methods. Based on the number of bands the of the medicinal plants were grouped to form1-4 clusters.
机译：用随机扩增多态性DNA（RAPD）标记分析了十八种药用植物之间的遗传关系，该标记可区分个体，并反映药用植物之间的固有变异和相互关系。本研究使用了13个decamer RAPD引物。 RAPD引物产生了超过77.23条可复制的条带，其中鉴定出77.23条多态性条带，赋予97.6％的多态性。所有的引物在每种药用植物中均产生典型的条带，这表明该试验在药用植物鉴定中的适用性。测试的大多数个体表现出具有独特的分子基因型。这些物种的种群遗传结构分析进一步揭示了高遗传分化系数（Gst），当第一个簇与其他药用植物配对时，种群之间的杂合性（Ht）与低基因流（Nm）一起显示。聚类分析的结果。使用欧几里得距离方法构建树状图。根据条带的数量，将药用植物的α-1分组为1-4簇。

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