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Evaluation of Rabbit Semen Quality Using Resazurin Reduction Test

机译:使用刃天青还原试验评估兔精液质量

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The resazurin reduction test (RRT) depends on the ability of metabolically active cells to reduce theresazurin dye to resorufin. In the current study, spectrophotometric evaluation of resazurin reduction test (RRT)to assess the color changes of resazurin reduction in butanol extracted color was used to evaluate rabbit semenquality.RRT was performed on one hundred rabbit semen samples in this investigation. The results of RRT weredetermined visually using resazurin colour chart and again the extent of resazurin reduction in each sample wasadditionally read by spectrophotometer to assess the quality of samples. Absorption was read at 580 nm and615 nm. Results indicated that RRT ratios decreased as the preservation time increased. and the highestcorrelation was observed with sperm motility (r=0.975, P<0.0001) and acrosomal integrity (r=0.864, P<0.0001).In conclusion, RRT could be used as a tool for evaluating the quality of rabbit semen.
机译:刃天青素减少试验(RRT)取决于代谢活性细胞将刃天青素染料还原为刃天青的能力。本研究使用分光光度法测定刃天青还原试验(RRT)来评估丁醇提取的颜色中刃天青还原的颜色变化,以评估兔精液质量.RRT在本次研究中对100个兔精液样品进行了RRT。使用刃天青素色图目测确定RRT的结果,并再次通过分光光度计读取每个样品中刃天青的还原程度,以评估样品的质量。在580nm和615nm处读取吸收。结果表明,随着保存时间的增加,RRT比率降低。结果表明,RRT可作为评价家兔精液质量的工具。最高的相关性是精子活力(r = 0.975,P <0.0001)和顶体完整性(r = 0.864,P <0.0001)。

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