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首页> 外文期刊>Global Veterinaria >Detection of Health Hazard - Food Born Viruses in Animal Products Anticipated for Human Consumption
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Detection of Health Hazard - Food Born Viruses in Animal Products Anticipated for Human Consumption

机译：检测预期危害人类消费的动物产品中的健康危害-食品中携带的病毒

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The contemporary study tries to launch a sensible, less expensive and precise method for detectingsome health hazard- food born viruses such as Foot and mouth disease virus (FMDV), Hepatitis A virus (HAV)and Rotavirus (RV) in animal products intended for human consumption in different localities. Samples fromfood (100 of each product at each locality) of animal origin were collected from 4 randomly localities- nearbyCairo, processed and virologically examined using Dot ELISA test, Monoplex RT-PCR and Electron microscopy.Examination revealed the existence of FMD, HVA and RV viruses in meat products (minced meat, beef burgerand sausage) as well as in milk and its products (ice-cream and cottage cheese). For all the viruses, RT-PCRsignificantly (P< 0.01) detect higher incidence than Dot ELISA in all the examined samples. The incidence ofFMDV is high in milk and its products, while, HAV is high in minced meat and RV is high in ice- cream. FMD,showed significant (P<0.01) locality variations for milk and its products only, while, HAV and RV virusesrevealed significant (P<0.01) locality variations in all the investigated products. Electron microscopy, showedsmall rounded virons (20-30 nm in size), resembling both FMD and HAV and larger spherical virons (80 nm)which resembling RV. It was concluded that FMD, HAV and RV viruses are present in animal products intendedfor human consumption and represent a source of health hazards. It is recommended to use RT-PCR fordetection of food born viruses as it is very sensitive, rapid and accurate method.
机译：这项当代研究试图推出一种明智，廉价且精确的方法，以检测用于人类的动物产品中某些危害健康的食品-天生病毒，如口蹄疫病毒（FMDV），甲型肝炎病毒（HAV）和轮状病毒（RV）各地消费。从开罗附近的4个随机地点采集动物源性食品的样本（每个地点的每种产品100个），进行处理并使用Dot ELISA试验，Monoplex RT-PCR和电​​子显微镜进行病毒学检查。检查发现存在FMD，HVA和RV肉制品（碎肉，牛肉汉堡和香肠）以及牛奶及其制品（冰淇淋和干酪）中的病毒。对于所有病毒，在所有检测的样品中，RT-PCR的发生率均显着高于Dot ELISA（P <0.01）。牛奶及其制品中FMDV的发生率很高，而碎肉中的HAV较高，冰淇淋中的RV较高。 FMD仅显示出牛奶及其产品的显着（P <0.01）局部变化，而HAV和RV病毒在所有研究产品中均显示出显着（P <0.01）局部变化。电子显微镜显示，圆形小维龙（大小为20-30 nm），类似于FMD和HAV;球形大维龙（80 nm），类似RV。结论是，FMD，HAV和RV病毒存在于供人类食用的动物产品中，并构成健康危害源。建议使用RT-PCR检测食源性病毒，因为它非常灵敏，快速且准确。

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