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首页> 外文期刊>Global Veterinaria >Comparison Between Enzyme Linked Immunosorbent Assay (ELISA) and Modified Agglutination Test (MAT) for Detection of Toxoplasma gondii Infection in Sheep and Goats Slaughtered in an Export Abattoir at Debre-zeit, Ethiopia
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Comparison Between Enzyme Linked Immunosorbent Assay (ELISA) and Modified Agglutination Test (MAT) for Detection of Toxoplasma gondii Infection in Sheep and Goats Slaughtered in an Export Abattoir at Debre-zeit, Ethiopia

机译：酶联免疫吸附测定（ELISA）和改良凝集试验（MAT）在埃塞俄比亚德布勒特地区屠宰出口屠宰场的绵羊和山羊中检测弓形虫感染的比较

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Toxoplasmosis is one of the most common parasitic zoonoses world-wide. Studies on prevalence andcomparison of the serodiagnosis of T. gondii infection in small ruminants in African countries are limited.A cross-sectional study was carried out from September, 2011 to November, 2012 on 242 cardiac blood samplesof sheep and goats collected from an export abattoir in Debre-Zeit, Central Ethiopia. Comparative study wasdone in diagnosis of T. gondii infection and revealed MAT = 27.7% (67/242) and ELISA 9.9% (24/242)seropositivity by Enzyme Linked Immunosorbent Assay (ELISA) and MAT (reference test) respectively witha moderate agreement (kappa = 0.42, P < 0.001). The overall sensitivity, specificity, positive and negativepredictive values of ELISA was 34.3%, 99.4%, 95.8% and 79.8% respectively. All ELISA positive samples werealso positive by MAT. The performance of ELISA is better in sheep than goat samples and in adult sheep andgoat samples than in younger. The high specificity and positive predictive value of ELISA indicate itspotential as diagnostic tests, particularly in areas with a high prevalence of infection while its low sensitivityand negative predictive value limit its value as a screening test. Therefore, MAT is reliable test for populationscreening tests. Further detailed evaluation of performance of ELISA as diagnostic test in various body fluids,different animal species and breeds is suggested.
机译：弓形虫病是世界范围内最常见的寄生性人畜共患病之一。在非洲国家的小反刍动物中，刚地弓形虫感染的血清学诊断和流行病学研究十分有限.2011年9月至2012年11月，对从出口屠宰场采集的242只绵羊和山羊的心脏血液样本进行了横断面研究在埃塞俄比亚中部的德布勒-泽特。一项比较研究在诊断弓形虫感染中完成了一项工作，并通过酶联免疫吸附测定（ELISA）和MAT（参考试验）分别以中等程度的同意率（MAT）检测了MAT = 27.7％（67/242）和ELISA 9.9％（24/242）血清阳性。 κ= 0.42，P <0.001）。 ELISA的总体灵敏度，特异性，阳性和阴性预测值分别为34.3％，99.4％，95.8％和79.8％。所有ELISA阳性样品也通过MAT呈阳性。在绵羊中，ELISA的性能要好于山羊，在成年绵羊和山羊中，ELISA的性能要比年轻的好。 ELISA的高特异性和阳性预测值表明了其作为诊断测试的潜力，特别是在感染流行率较高的地区，而ELISA的低灵敏度和阴性预测值限制了其作为筛选测试的价值。因此，MAT是用于人群筛选测试的可靠测试。建议进一步详细评估ELISA在各种体液，不同动物物种和品种中作为诊断测试的性能。

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