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首页> 外文期刊>Gut Pathogens >Presumable role of outer membrane proteins of Salmonella containing sialylated lipopolysaccharides serovar Ngozi, sv. Isaszeg and subspecies arizonae in determining susceptibility to human serum
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Presumable role of outer membrane proteins of Salmonella containing sialylated lipopolysaccharides serovar Ngozi, sv. Isaszeg and subspecies arizonae in determining susceptibility to human serum

机译：沙门氏菌含有唾液酸化脂多糖血清型Ngozi，sv的外膜蛋白的推测作用。 Isaszeg和亚种Arizonae在确定对人血清的敏感性时

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Background The O48 group comprises Salmonella bacteria containing sialic acid in the lipopolysaccharide (LPS). Bacteria with sialylated surface structures are described as pathogens that avoid immunological response of the host by making similar their surface antigens to the host’s tissues (molecular mimicry). It is known that the smooth-type LPS of Salmonella enterica and outer membrane proteins (OMP) PgtE, PagC and Rck mediate serum resistant phenotype by affecting complement system (C). The aim of this study was to investigate C3 component activation by Salmonella O48 LPS and OMP. Findings In the present study, we examined C3 component deposition on the three Salmonella O48 strains: S. enterica subspecies enterica serovar Ngozi, S. enterica subsp. enterica sv. Isaszeg, and S. enterica subsp. arizonae containing sialic acid in the O-specific part of LPS. The greatest C3 deposition occurred on Salmonella sv. Isaszeg cells (p < 0.005) as well as on their LPS (low content of sialic acid in LPS) (p < 0.05) after 45 min of incubation in 50% human serum. Weaker C3 deposition ratio on the Salmonella sv. Ngozi (high content of sialic acid in LPS) and Salmonella subsp. arizonae (high content of sialic acid in LPS) cells correlated with the lower C3 activation on their LPS. Immunoblotting revealed that OMP isolated from the tested strains also bound C3 protein fragments. Conclusions We suggest that activation of C3 serum protein is dependent on the sialic acid contents in the LPS as well as on the presence of OMP in the range of molecular masses of 35–48 kDa.
机译：背景O48基团包含沙门氏菌，脂多糖（LPS）中含有唾液酸。具有唾液酸化表面结构的细菌被描述为通过使宿主的表面抗原与宿主组织相似（分子模拟）而避免宿主免疫应答的病原体。已知肠炎沙门氏菌的平滑型LPS和外膜蛋白（OMP）PgtE，PagC和Rck通过影响补体系统（C）介导血清抗性表型。这项研究的目的是调查沙门氏菌O48 LPS和OMP激活C3成分。调查结果在本研究中，我们研究了三种沙门氏菌O48菌株上的C3成分沉积：肠炎沙门氏菌亚种肠炎沙门氏菌Ngozi，肠炎沙门氏菌亚种。 Enterica sv。 Isaszeg和S. enterica subsp。在LPS的O特异部分中含有唾液酸的亚利桑那州。最大的C3沉积发生在沙门氏菌上。在50％人血清中孵育45分钟后，Isaszeg细胞（p <0.005）以及其LPS（LPS中唾液酸含量低）（p <0.05）。沙门氏菌上C3的沉积率较弱。 Ngozi（LPS中唾液酸含量高）和沙门氏菌亚种。亚利桑那州（LPS中唾液酸含量高）细胞与其LPS上较低的C3活化有关。免疫印迹显示，从测试菌株中分离出的OMP也结合了C3蛋白片段。结论我们建议C3血清蛋白的激活取决于LPS中唾液酸的含量以及35-48 kDa分子量范围内OMP的存在。

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