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首页> 外文期刊>Folia histochemica et cytobiologica >Characteristics of weak base-induced vacuoles formed around individual acidic organelles
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Characteristics of weak base-induced vacuoles formed around individual acidic organelles

机译：弱酸性诱导的液泡在单个酸性细胞器周围形成的特征

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We have previously found that the weak base 4-aminopyridine induces Brownian motion of acidic organelles around which vacuoles are formed, causing organelle traffic disorder in neurons. Our present study investigated the characteristics of vacuoles induced by weak bases (NH 4 Cl, aminopyridines, and chloroquine) using mouse cells. Individual vacuoles included acidic organelles identified by fluorescent protein expression. Mitochondria and actin filaments were extruded outside the vacuoles, composing the vacuole rim. Staining with amine-reactive fluorescence showed no protein/amino acid content in vacuoles. Thus, serous vacuolar contents are probably partitioned by viscous cytosol, other organelles, and cytoskeletons, but not membrane. The weak base (chloroquine) was immunochemically detected in intravacuolar organelles, but not in vacuoles. Early vacuolization was reversible, but long-term vacuolization caused cell death. The vacuolization and cell death were blocked by the vacuolar H + -ATPase inhibitor and Cl–-free medium. Staining with LysoTracker or LysoSensor indicated that intravacuolar organelles were strongly acidic and vacuoles were slightly acidic. This suggests that vacuolization is caused by accumulation of weak base and H + in acidic organelles, driven by vacuolar H + -ATPase associated with Cl – entering, and probably by subsequent extrusion of H + and water from organelles to the surrounding cytoplasm. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 272–279)
机译：我们以前已经发现弱碱4-氨基吡啶诱导酸性细胞器的布朗运动，在其周围形成液泡，从而导致神经元中的细胞器运输障碍。我们的研究调查了使用小鼠细胞由弱碱（NH 4 Cl，氨基吡啶和氯喹）诱导的液泡特征。单个液泡包括通过荧光蛋白表达鉴定的酸性细胞器。将线粒体和肌动蛋白丝挤出到液泡的外面，组成液泡的边缘。用胺反应性荧光染色显示液泡中没有蛋白质/氨基酸含量。因此，浆液中的液泡内容物可能被粘性的细胞溶胶，其他细胞器和细胞骨架分配，但没有被膜分配。弱碱（氯喹）是通过免疫化学方法在真空细胞器中检测到的，而在液泡中则没有。早期空泡化是可逆的，但长期空泡化会导致细胞死亡。空泡和细胞死亡被液泡H + -ATPase抑制剂和无Cl-的培养基阻止。用LysoTracker或LysoSensor染色表明，真空中的细胞器是强酸性的，液泡是弱酸性的。这表明空泡化是由酸性细胞器中弱碱和H +的积累引起的，其由与Cl-结合的液泡H + -ATPase进入驱动，并且可能是随后H +和水从细胞器向周围细胞质的挤出。 （Folia Histochemica et Cytobiologica 2011;第49卷，第2期，第272-279页）

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