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首页> 外文期刊>Folia histochemica et cytobiologica >Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS
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Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS

机译：人脐带表达几种参与血管张力局部调节的血管活性肽：孤儿蛋白，催产素，ANP，eNOS和iNOS的蛋白质和基因表达

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Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. The aim of this study was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). The expression of these molecules in full-term human umbilical cord was investigated through immunohistochemistry and RT-PCR. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in the human umbilical cord. mRNA expression for Oxytocin, ANP and eNOS suggest that these molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism locally regulating vascular tone. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 211–218)
机译：完整的人类脐带包含三条血管：两条动脉缠绕在静脉周围，并被沃顿氏胶包裹，粘液组织几乎没有间质基质细胞和丰富的细胞外基质。脐带血管缺乏神经支配，因此内皮细胞必须在控制血流中发挥作用。这项研究的目的是研究人脐带中可能参与调节血管张力的五种肽的表达：孤儿蛋白FQ，催产素，心钠素，内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮氧化物合酶（iNOS）。通过免疫组织化学和RT-PCR研究了这些分子在足月人脐带中的表达。在沃顿商学院的果冻，血管肌肉组织和内皮细胞中检测到了孤儿蛋白FQ的免疫反应性。催产素，ANP和eNOS由脐带上皮，沃顿氏胶和内皮表达，而iNOS仅由内皮细胞表达。 RT-PCR分析显示催产素，ANP和eNOS mRNA的转录表达。在人的脐带中发现了orphanin，催产素，ANP，eNOS和iNOS蛋白的存在。催产素，ANP和eNOS的mRNA表达表明这些分子是由脐带细胞自身合成的。这些血管活性分子的表达可能是局部调节血管紧张度的一般机制的一部分。 （Folia Histochemica et Cytobiologica 2011;第49卷，第2期，第211-218页）

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