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首页> 外文期刊>Folia histochemica et cytobiologica >The effect of octreotide and bromocriptine on expression of a pro-apoptotic Bax protein in rat prolactinoma.
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The effect of octreotide and bromocriptine on expression of a pro-apoptotic Bax protein in rat prolactinoma.

机译：奥曲肽和溴隐亭对大鼠催乳素瘤中促凋亡Bax蛋白表达的影响。

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It is well established that disruption of apoptosis may lead to tumor initiation, progression or metastasis. It is also well documented that many anticancer drugs induce apoptosis. In the earlier studies, the dopamine D2 receptor agonist bromocriptine (BC) and somatostatin analog octreotide (OCT) were found to inhibit the growth of the estrogen-induced rat prolactinoma. Our previous investigations, applying the TUNEL method showed the involvement of the pro-apoptotic effect in the action of BC, and to a lesser degree, in the action of OCT. The aim of the present study was to investigate whether the pro-apoptotic action of these drugs involves the increased expression of Bax--a member of Bcl-2 protein family which is known to play an important role in the regulation of apoptosis. Male four-week Fisher 344 rats were used in the experiment. Capsules containing diethylstilboestrol (DES) were implanted subcutaneously. Six weeks after the implantation the rats were given OCT (2 x 25 microg/animal/24), BC (3 mg/kg b.w./24 h) or OCT and BC at the above doses for 10 days. Bax expression was detected by immunohistochemistry. Prolactin (PRL) in blood serum was measured by radioimmunoassay (RIA). It has been found that both OCT and BC, alone or in combination, significantly reduce the tumor weight. Both OCT and BC suppressed PRL levels, but the inhibitory effect of BC was stronger than that of OCT. It has been found that the treatment with OCT and BC, alone or in combination, causes a significant increase in Bax expression in the rat prolactinoma cells. Our findings indicate that anti-tumoral action of bromocriptine and to some extent the action of octreotide in the experimental rat prolactinoma is connected with the induction of apoptosis and is associated with increased Bax expression.
机译：众所周知，凋亡的破坏可能导致肿瘤的发生，进展或转移。也有充分的文献证明许多抗癌药物诱导细胞凋亡。在较早的研究中，发现多巴胺D2受体激动剂溴隐亭（BC）和生长抑素类似物奥曲肽（OCT）抑制雌激素诱导的大鼠催乳素瘤的生长。我们之前使用TUNEL方法进行的研究表明，促凋亡作用与BC的作用有关，而OCT的作用较小。本研究的目的是研究这些药物的促凋亡作用是否涉及Bax的表达增加-Bax是Bcl-2蛋白家族的一个成员，已知该家族在调节细胞凋亡中起着重要作用。实验中使用雄性四周费舍尔344只大鼠。含有二乙基雌二醇（DES）的胶囊被皮下植入。植入后六周，给大鼠以上述剂量的OCT（2 x 25 microg /动物/ 24），BC（3 mg / kg b.w./24 h）或OCT和BC接种10天。通过免疫组织化学检测Bax表达。通过放射免疫测定法（RIA）测定血清中的催乳激素（PRL）。已经发现，单独或组合使用OCT和BC均可显着降低肿瘤重量。 OCT和BC均可抑制PRL水平，但BC的抑制作用强于OCT。已经发现，单独或组合使用OCT和BC进行的治疗引起大鼠催乳素瘤细胞中Bax表达的显着增加。我们的发现表明，溴隐亭的抗肿瘤作用以及奥曲肽在实验大鼠催乳素瘤中的某种作用与细胞凋亡的诱导有关，并与增加的Bax表达有关。

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